54750

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 5809..6334 Replicon plasmid pKPC_CAV1596-97
Accession NZ_CP011646
Organism Klebsiella pneumoniae strain CAV1596

Toxin (Protein)


Gene name ccdB Uniprot ID W8UQV6
Locus tag AB187_RS00715 Protein ID WP_001568026.1
Coordinates 5809..6114 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag AB187_RS00720 Protein ID WP_001568025.1
Coordinates 6116..6334 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AB187_RS00685 1509..2135 + 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -
AB187_RS29930 2255..2434 + 180 WP_006788217.1 hypothetical protein -
AB187_RS00695 2890..3684 - 795 WP_004197635.1 site-specific integrase -
AB187_RS00700 3882..4898 - 1017 WP_020315256.1 hypothetical protein -
AB187_RS00705 4895..5218 - 324 WP_022644730.1 hypothetical protein -
AB187_RS00710 5245..5640 - 396 WP_017899885.1 hypothetical protein -
AB187_RS00715 5809..6114 - 306 WP_001568026.1 type II toxin-antitoxin system toxin CcdB Toxin
AB187_RS00720 6116..6334 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
AB187_RS00725 6887..7576 + 690 WP_004193995.1 hypothetical protein -
AB187_RS00730 7608..8297 - 690 WP_020315560.1 RES family NAD+ phosphorylase -
AB187_RS00735 8734..9003 + 270 WP_032743927.1 hypothetical protein -
AB187_RS00740 8991..9566 + 576 WP_020317495.1 hypothetical protein -
AB187_RS00745 9597..10091 + 495 WP_020802777.1 hypothetical protein -
AB187_RS00750 10135..10503 + 369 WP_004197807.1 hypothetical protein -
AB187_RS00755 10537..10740 + 204 WP_004197808.1 hemolysin expression modulator Hha -
AB187_RS00760 10754..10957 + 204 WP_004197809.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaKPC-3 / blaSHV-12 - 1..96702 96702


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11645.32 Da        Isoelectric Point: 5.6919

>T54750 WP_001568026.1 NZ_CP011646:c6114-5809 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASAWLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T54750 NZ_CP011646:c6114-5809 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCTGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT54750 WP_001568025.1 NZ_CP011646:c6334-6116 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT54750 NZ_CP011646:c6334-6116 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGTGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A514EZN1

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References