Detailed information of TA system TA054749

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/Xre(antitoxin)
• Location: 8885..9374
• Replicon: plasmid pKPC_CAV1596-78
• Accession: NZ_CP011645
• Organism: Klebsiella pneumoniae strain CAV1596

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: U3PL64
• Locus tag: AB187_RS00315
• Protein ID: WP_020314676.1
• Coordinates: 8885..9145 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: -
• Locus tag: AB187_RS28510
• Protein ID: WP_022540736.1
• Coordinates: 9135..9374 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AB187_RS28500	4973..5302	+	330	WP_001542022.1	hypothetical protein	-
AB187_RS00280	5706..6221	+	516	WP_001025393.1	J domain-containing protein	-
AB187_RS00285	6325..6867	+	543	WP_001083904.1	hypothetical protein	-
AB187_RS00290	6864..7118	+	255	WP_001230709.1	hypothetical protein	-
AB187_RS00295	7115..7357	+	243	WP_000151456.1	hypothetical protein	-
AB187_RS00305	7938..8261	+	324	WP_020314677.1	hypothetical protein	-
AB187_RS00310	8303..8512	+	210	WP_020314678.1	hypothetical protein	-
AB187_RS28505	8609..8833	+	225	WP_000814381.1	hypothetical protein	-
AB187_RS00315	8885..9145	+	261	WP_020314676.1	hypothetical protein	Toxin
AB187_RS28510	9135..9374	+	240	WP_022540736.1	transcriptional regulator	Antitoxin
AB187_RS00325	9909..10169	+	261	WP_020314977.1	hypothetical protein	-
AB187_RS00330	10556..10738	-	183	WP_001401700.1	hypothetical protein	-
AB187_RS28520	11283..11465	+	183	WP_000833470.1	type II toxin-antitoxin system HicA family toxin	-
AB187_RS28525	11490..11927	+	438	WP_000466318.1	type II toxin-antitoxin system HicB family antitoxin	-
AB187_RS00335	12058..12378	-	321	WP_001310435.1	hypothetical protein	-
AB187_RS00340	12375..12890	-	516	WP_001229189.1	hypothetical protein	-
AB187_RS00345	12927..13190	-	264	WP_020314988.1	hypothetical protein	-
AB187_RS00350	13192..13611	-	420	WP_001708405.1	hypothetical protein	-
AB187_RS00355	13631..14338	-	708	WP_000108796.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaKPC-3 / aac(6')-Ib / ant(3'')-Ia / blaOXA-9 / blaTEM-1A	-	1..77801	77801

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (8-37)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9922.30 Da
• Isoelectric Point: 7.2028

>T54749 WP_020314676.1 NZ_CP011645:8885-9145 [Klebsiella pneumoniae]
MKIRKGDRQYYLNKEGDTFHLVKRVKTFSKSATLGKTKATVKTVADLVFHEKAFDTIDFASDGLRDNDKEIVFMMIQEMS
EEENAN

Nucleotide

• Length: 261 bp

>T54749 NZ_CP011645:8885-9145 [Klebsiella pneumoniae]
ATGAAGATAAGAAAAGGTGACAGACAATATTATCTGAATAAAGAAGGTGATACGTTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAGTCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCTGATCTTGTTTTTCATGAGAAGGCAT
TCGACACTATCGACTTTGCCAGCGACGGATTAAGAGATAATGATAAAGAAATTGTTTTTATGATGATTCAGGAAATGAGC
GAAGAAGAAAATGCTAACTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9072.61 Da
• Isoelectric Point: 10.2613

>AT54749 WP_022540736.1 NZ_CP011645:9135-9374 [Klebsiella pneumoniae]
MLTENTPENIKRLRKKIGLTQKECAEIFSMSPRTWRRKEEPVGTASGTALTPVEFKYLLLLAGEHPEYVLSKRDRGILK

Nucleotide

• Length: 240 bp

>AT54749 NZ_CP011645:9135-9374 [Klebsiella pneumoniae]
ATGCTAACTGAAAACACACCAGAAAACATTAAGCGTCTGCGGAAAAAAATTGGCCTGACACAGAAAGAATGTGCCGAAAT
ATTCAGCATGAGTCCGCGTACATGGCGTCGTAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTTACACCCGTGG
AGTTTAAATATCTTCTGCTTTTAGCTGGGGAACATCCTGAATACGTCCTTAGTAAAAGAGATCGAGGAATTTTAAAATGA

Structures

Toxin

• AlphaFold DB: A0A376MG86