Detailed information of TA system TA054712

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2131478..2132103
• Replicon: chromosome
• Accession: NZ_CP011624
• Organism: Klebsiella pneumoniae strain CAV1344

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AB186_RS13670
• Protein ID: WP_047718441.1
• Coordinates: 2131478..2131861 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: J2DFR0
• Locus tag: AB186_RS13675
• Protein ID: WP_004150355.1
• Coordinates: 2131861..2132103 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AB186_RS13655	2128844..2129746	+	903	WP_002882822.1	formate dehydrogenase subunit beta	-
AB186_RS13660	2129743..2130378	+	636	WP_002882818.1	formate dehydrogenase cytochrome b556 subunit	-
AB186_RS13665	2130375..2131304	+	930	WP_004150358.1	formate dehydrogenase accessory protein FdhE	-
AB186_RS13670	2131478..2131861	-	384	WP_047718441.1	type II toxin-antitoxin system VapC family toxin	Toxin
AB186_RS13675	2131861..2132103	-	243	WP_004150355.1	ribbon-helix-helix domain-containing protein	Antitoxin
AB186_RS13680	2132308..2133225	+	918	WP_004187175.1	alpha/beta hydrolase	-
AB186_RS13685	2133239..2134180	-	942	WP_004178031.1	fatty acid biosynthesis protein FabY	-
AB186_RS13690	2134225..2134662	-	438	WP_002882809.1	D-tyrosyl-tRNA(Tyr) deacylase	-
AB186_RS13695	2134659..2135519	-	861	WP_004146232.1	virulence factor BrkB family protein	-
AB186_RS13700	2135513..2136112	-	600	WP_004151865.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (16-47)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14350.59 Da
• Isoelectric Point: 7.3178

>T54712 WP_047718441.1 NZ_CP011624:c2131861-2131478 [Klebsiella pneumoniae]
MTSGSALFDTSILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Nucleotide

• Length: 384 bp

>T54712 NZ_CP011624:c2131861-2131478 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAGTATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9567.83 Da
• Isoelectric Point: 4.3509

>AT54712 WP_004150355.1 NZ_CP011624:c2132103-2131861 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Nucleotide

• Length: 243 bp

>AT54712 NZ_CP011624:c2132103-2131861 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GGU9