Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 180988..181724 Replicon plasmid pCAV1344-250
Accession NZ_CP011623
Organism Klebsiella pneumoniae strain CAV1344

Toxin (Protein)


Gene name tacT Uniprot ID -
Locus tag AB186_RS02740 Protein ID WP_038992631.1
Coordinates 180988..181470 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID -
Locus tag AB186_RS02745 Protein ID WP_032415032.1
Coordinates 181458..181724 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AB186_RS02725 176563..178275 + 1713 WP_038992633.1 ROK family protein -
AB186_RS02730 178723..179355 + 633 WP_001567369.1 hypothetical protein -
AB186_RS02735 179384..180787 - 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
AB186_RS02740 180988..181470 - 483 WP_038992631.1 GNAT family N-acetyltransferase Toxin
AB186_RS02745 181458..181724 - 267 WP_032415032.1 DUF1778 domain-containing protein Antitoxin
AB186_RS02750 182169..182399 - 231 WP_004206660.1 hypothetical protein -
AB186_RS02755 182413..182616 - 204 WP_004206661.1 hemolysin expression modulator Hha -
AB186_RS02760 182677..183168 - 492 WP_004206662.1 hypothetical protein -
AB186_RS02765 183302..184513 - 1212 WP_032415728.1 hypothetical protein -
AB186_RS02770 184510..184758 - 249 WP_004193994.1 hypothetical protein -
AB186_RS02775 185237..185914 + 678 WP_047718105.1 helix-turn-helix domain-containing protein -
AB186_RS02780 185995..186405 + 411 WP_047718088.1 heme-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..250396 250396
- flank IS/Tn - - 179384..180787 1403


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(66-149)

Antitoxin

Vibrio phage ICP1, Orf50

(2-87)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17351.06 Da        Isoelectric Point: 10.0704

>T54708 WP_038992631.1 NZ_CP011623:c181470-180988 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKSSQTHERTLFLKLP

Download         Length: 483 bp

>T54708 NZ_CP011623:c181470-180988 [Klebsiella pneumoniae]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGAAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCTACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGTGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGCGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGTCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10123.53 Da        Isoelectric Point: 4.5141

>AT54708 WP_032415032.1 NZ_CP011623:c181724-181458 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT54708 NZ_CP011623:c181724-181458 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCCCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGATTCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References