Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 81347..81873 Replicon plasmid pCAV1344-250
Accession NZ_CP011623
Organism Klebsiella pneumoniae strain CAV1344

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag AB186_RS02210 Protein ID WP_000323025.1
Coordinates 81586..81873 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID J5W3H0
Locus tag AB186_RS02205 Protein ID WP_004196370.1
Coordinates 81347..81586 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AB186_RS31485 76684..76876 - 193 Protein_87 MFS transporter -
AB186_RS02180 76906..77883 + 978 WP_004196334.1 chromate resistance protein -
AB186_RS31490 77840..79216 + 1377 Protein_89 chromate efflux transporter -
AB186_RS02190 79247..79936 - 690 WP_004196322.1 hypothetical protein -
AB186_RS02195 79950..80687 - 738 WP_008460272.1 HupE/UreJ family protein -
AB186_RS29820 80731..81096 - 366 WP_009651956.1 hypothetical protein -
AB186_RS02205 81347..81586 + 240 WP_004196370.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
AB186_RS02210 81586..81873 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
AB186_RS02215 81940..82974 - 1035 Protein_95 IS481-like element ISKpn28 family transposase -
AB186_RS02220 83046..83204 + 159 WP_014343509.1 type I toxin-antitoxin system Hok family toxin -
AB186_RS02225 83283..84395 + 1113 WP_001300563.1 IS4-like element IS421 family transposase -
AB186_RS02230 85251..85523 + 273 WP_032425552.1 hypothetical protein -
AB186_RS02235 85520..85870 + 351 WP_032425551.1 hypothetical protein -
AB186_RS02240 85885..86202 + 318 WP_015065519.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..250396 250396


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(3-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T54707 WP_000323025.1 NZ_CP011623:81586-81873 [Klebsiella pneumoniae]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T54707 NZ_CP011623:81586-81873 [Klebsiella pneumoniae]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9018.43 Da        Isoelectric Point: 4.3894

>AT54707 WP_004196370.1 NZ_CP011623:81347-81586 [Klebsiella pneumoniae]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLCNPKPVRVTLDEL

Download         Length: 240 bp

>AT54707 NZ_CP011623:81347-81586 [Klebsiella pneumoniae]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTTGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4QRH4

References