Detailed information of TA system TA054694

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 5522479..5523117
• Replicon: chromosome
• Accession: NZ_CP011618
• Organism: Klebsiella oxytoca strain CAV1335

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: AB181_RS28360
• Protein ID: WP_047043763.1
• Coordinates: 5522935..5523117 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: AB181_RS28355
• Protein ID: WP_047720836.1
• Coordinates: 5522479..5522910 (-)
• Length: 144 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AB181_RS28335	5517595..5517852	+	258	WP_004122994.1	hypothetical protein	-
AB181_RS28340	5517852..5518736	+	885	WP_047720833.1	phage N-6-adenine-methyltransferase	-
AB181_RS28345	5518729..5520576	+	1848	WP_047720834.1	DNA cytosine methyltransferase	-
AB181_RS28350	5520573..5521634	+	1062	WP_047720835.1	DUF968 domain-containing protein	-
AB181_RS33120	5521647..5522249	+	603	WP_071845044.1	hypothetical protein	-
AB181_RS28355	5522479..5522910	-	432	WP_047720836.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
AB181_RS28360	5522935..5523117	-	183	WP_047043763.1	type II toxin-antitoxin system HicA family toxin	Toxin
AB181_RS28365	5523359..5523688	+	330	WP_139153342.1	phage holin, lambda family	-
AB181_RS28370	5523675..5524094	+	420	WP_047720838.1	structural protein	-
AB181_RS28375	5524097..5524564	+	468	WP_047720839.1	lysis protein	-
AB181_RS28380	5524795..5525025	+	231	WP_047720840.1	hypothetical protein	-
AB181_RS34215	5525809..5526066	-	258	WP_139153332.1	hypothetical protein	-
AB181_RS28385	5526523..5526708	+	186	WP_047720841.1	hypothetical protein	-
AB181_RS28390	5526799..5527131	+	333	WP_047720842.1	hypothetical protein	-
AB181_RS28395	5527131..5527550	+	420	WP_047720843.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	5493053..5569551	76498

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-60)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (7-137)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6508.74 Da
• Isoelectric Point: 10.8930

>T54694 WP_047043763.1 NZ_CP011618:c5523117-5522935 [Klebsiella oxytoca]
MKSTDLIKELIAAGCELKRQGKGSHQIWWSPITGKTFPVPHPKGNLPIGTIKSIKKTAGI

Nucleotide

• Length: 183 bp

>T54694 NZ_CP011618:c5523117-5522935 [Klebsiella oxytoca]
ATGAAATCGACTGACCTGATAAAGGAATTGATAGCCGCCGGATGTGAACTCAAAAGGCAAGGGAAAGGCAGTCACCAGAT
ATGGTGGTCGCCGATAACGGGAAAAACATTCCCGGTTCCACACCCTAAGGGCAACCTACCGATCGGCACCATCAAATCCA
TCAAGAAAACGGCGGGGATCTAA

Antitoxin

Protein

• Length: 144 a.a.
• Molecular weight: 16029.07 Da
• Isoelectric Point: 4.2696

>AT54694 WP_047720836.1 NZ_CP011618:c5522910-5522479 [Klebsiella oxytoca]
MFFSVGVETPKDENTAYGMIVPAFSAYEMGCFSAADTQDQIAPMVKEAILLSVECLVEDAGRSVDEIKDAGYMVYAANPE
YKDFDSWFMVEVDLSEFEGKPQRINISLPDTLIKRIDNAVKGNASYRDRSHFLAEAARHELKQ

Nucleotide

• Length: 432 bp

>AT54694 NZ_CP011618:c5522910-5522479 [Klebsiella oxytoca]
ATGTTCTTTTCAGTGGGCGTTGAGACGCCAAAAGATGAAAACACCGCCTACGGTATGATTGTTCCTGCGTTTTCAGCTTA
TGAGATGGGTTGCTTCTCCGCCGCTGATACACAGGACCAGATCGCGCCAATGGTTAAAGAAGCCATTCTCTTAAGCGTTG
AGTGCCTGGTCGAAGATGCAGGGCGCAGCGTCGATGAAATCAAAGACGCCGGCTATATGGTGTATGCAGCTAACCCAGAA
TATAAGGACTTTGACAGCTGGTTCATGGTTGAAGTTGATTTGTCTGAGTTCGAAGGCAAGCCACAACGTATCAATATTTC
ACTGCCTGATACGCTGATTAAGCGCATCGATAATGCTGTAAAGGGTAACGCCAGCTATCGAGACAGAAGCCATTTTCTGG
CTGAAGCTGCCCGACACGAACTGAAGCAGTGA