Detailed information of TA system TA054690

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 2165944..2166563
• Replicon: chromosome
• Accession: NZ_CP011618
• Organism: Klebsiella oxytoca strain CAV1335

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: H3N9D8
• Locus tag: AB181_RS12560
• Protein ID: WP_004099646.1
• Coordinates: 2166345..2166563 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: AB181_RS12555
• Protein ID: WP_004099648.1
• Coordinates: 2165944..2166318 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AB181_RS12545	2161100..2162293	+	1194	WP_004111040.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
AB181_RS12550	2162316..2165462	+	3147	WP_004099650.1	multidrug efflux RND transporter permease subunit	-
AB181_RS12555	2165944..2166318	+	375	WP_004099648.1	Hha toxicity modulator TomB	Antitoxin
AB181_RS12560	2166345..2166563	+	219	WP_004099646.1	hemolysin expression modulator Hha	Toxin
AB181_RS12565	2166724..2167290	+	567	WP_004099644.1	maltose O-acetyltransferase	-
AB181_RS12570	2167427..2167897	+	471	WP_004111038.1	YlaC family protein	-
AB181_RS12575	2167872..2169326	-	1455	WP_024273853.1	PLP-dependent aminotransferase family protein	-
AB181_RS12580	2169429..2170127	+	699	WP_004099639.1	GNAT family N-acetyltransferase	-
AB181_RS32435	2170124..2170264	-	141	WP_003859006.1	type B 50S ribosomal protein L36	-
AB181_RS12585	2170264..2170527	-	264	WP_004099638.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8640.05 Da
• Isoelectric Point: 8.9008

>T54690 WP_004099646.1 NZ_CP011618:2166345-2166563 [Klebsiella oxytoca]
MSDKTLTKIDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWKFIR

Nucleotide

• Length: 219 bp

>T54690 NZ_CP011618:2166345-2166563 [Klebsiella oxytoca]
ATGTCTGATAAAACATTAACCAAAATAGACTATTTGATGCGCTTACGACGTTGTCAGACAATTGATACGCTGGAGCGCGT
AATTGAAAAAAATAAATATGAACTTTCTGATAATGAACTGGCGGTATTTTACTCAGCAGCCGATCATCGTCTTGCTGAAC
TGACGATGAATAAACTGTATGACAAGATCCCGCCATCGGTCTGGAAATTCATCCGCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14350.09 Da
• Isoelectric Point: 4.8989

>AT54690 WP_004099648.1 NZ_CP011618:2165944-2166318 [Klebsiella oxytoca]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDNKLVTQLDEYL
DDTFVLFSNYGINTADLQKWRKSGNRLFRCFVNASRENPASLSC

Nucleotide

• Length: 375 bp

>AT54690 NZ_CP011618:2165944-2166318 [Klebsiella oxytoca]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTTAATGACCCAACCTCTGCTGTCAATCTTCAGCTTAATGAATTGATCGAGC
ACATTGCCGCGTTCGCACTTAATTATAAAATTAAGTATGCTGAGGATAATAAGCTGGTGACTCAGCTTGACGAATATCTG
GATGACACTTTCGTGTTGTTCAGCAACTACGGCATTAACACTGCAGATCTGCAGAAATGGCGTAAATCAGGCAACAGGTT
ATTCCGCTGCTTTGTGAACGCCAGTCGGGAAAACCCGGCCAGTCTTTCTTGTTAA

Structures

Toxin

• AlphaFold DB: A0A0H3H713