Detailed information of TA system TA054689

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 68658..69394
• Replicon: plasmid pCAV1335-115
• Accession: NZ_CP011617
• Organism: Klebsiella oxytoca strain CAV1335

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: AB181_RS02215
• Protein ID: WP_003026803.1
• Coordinates: 68912..69394 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: AB181_RS02210
• Protein ID: WP_003026799.1
• Coordinates: 68658..68924 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AB181_RS02180	64125..65093	-	969	WP_077257929.1	IS5-like element IS903B family transposase	-
AB181_RS02185	65120..65350	+	231	Protein_69	oxidoreductase	-
AB181_RS31995	65581..65772	+	192	Protein_70	IS1 family transposase	-
AB181_RS02190	65824..66792	-	969	WP_176678774.1	IS5 family transposase	-
AB181_RS02195	67214..67705	+	492	WP_004206662.1	hypothetical protein	-
AB181_RS02200	67766..67969	+	204	WP_004213596.1	hemolysin expression modulator Hha	-
AB181_RS02205	67983..68213	+	231	WP_004206660.1	hypothetical protein	-
AB181_RS02210	68658..68924	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
AB181_RS02215	68912..69394	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
AB181_RS02220	69595..70998	+	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
AB181_RS02225	71027..71659	-	633	WP_001567369.1	hypothetical protein	-
AB181_RS02230	71734..72105	+	372	Protein_79	IS5/IS1182 family transposase	-
AB181_RS02235	72137..73105	+	969	WP_013815099.1	IS5-like element IS903B family transposase	-
AB181_RS02240	73266..74081	+	816	WP_012695470.1	aminoglycoside O-phosphotransferase APH(3')-Ia	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul1 / aadA2 / aph(3')-Ia / mcr-9 / aph(6)-Id / aph(3'')-Ib / dfrA19 / qnrB2	pla	1..115319	115319
	-	inside	IScluster/Tn	aph(3')-Ia	-	64125..79737	15612

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T54689 WP_003026803.1 NZ_CP011617:68912-69394 [Klebsiella oxytoca]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T54689 NZ_CP011617:68912-69394 [Klebsiella oxytoca]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT54689 WP_003026799.1 NZ_CP011617:68658-68924 [Klebsiella oxytoca]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT54689 NZ_CP011617:68658-68924 [Klebsiella oxytoca]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66