Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4408950..4409570 Replicon chromosome
Accession NZ_CP011612
Organism Citrobacter freundii strain CAV1321

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag AB180_RS23890 Protein ID WP_002892050.1
Coordinates 4408950..4409168 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag AB180_RS23895 Protein ID WP_003021733.1
Coordinates 4409196..4409570 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AB180_RS23860 4404183..4404812 + 630 WP_003835929.1 membrane protein -
AB180_RS23865 4404877..4405230 + 354 WP_003831033.1 DUF1428 family protein -
AB180_RS23870 4405282..4406835 - 1554 WP_044699371.1 EAL domain-containing protein -
AB180_RS23875 4407024..4407284 + 261 WP_003021719.1 type B 50S ribosomal protein L31 -
AB180_RS27940 4407286..4407426 + 141 WP_005125190.1 type B 50S ribosomal protein L36 -
AB180_RS23880 4407505..4407975 - 471 WP_003021724.1 YlaC family protein -
AB180_RS23885 4408092..4408643 - 552 WP_044699372.1 maltose O-acetyltransferase -
AB180_RS23890 4408950..4409168 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
AB180_RS23895 4409196..4409570 - 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
AB180_RS23900 4410058..4413207 - 3150 WP_003021736.1 multidrug efflux RND transporter permease subunit -
AB180_RS23905 4413230..4414423 - 1194 WP_003835922.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T54688 WP_002892050.1 NZ_CP011612:c4409168-4408950 [Citrobacter freundii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T54688 NZ_CP011612:c4409168-4408950 [Citrobacter freundii]
ATGTCCGATAAACCATTAACTAAAACAGATTATTTGATGCGCCTGCGACGTTGCCAGACAATTGATACGCTAGAGCGCGT
TATTGAAAAAAATAAATATGAATTGTCCGACAACGAACTGGCTGTATTTTACTCAGCAGCAGATCATCGCCTTGCTGAAT
TGACCATGAATAAGCTTTACGACAAGATTCCAACTTCTGTTTGGAAATTCATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT54688 WP_003021733.1 NZ_CP011612:c4409570-4409196 [Citrobacter freundii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT54688 NZ_CP011612:c4409570-4409196 [Citrobacter freundii]
ATGGATGAATACTCACCCAAAAGACATGATATCGCGCAGCTTAAATTTCTCTGCGAAACTCTGTATCATGACTGTCTTGC
AAACCTTGAACAAAGTAACCATGGCTGGGTTAATGACCCTACCTCTGCAACCAGCTTGCAGCTCAATGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAGGACAATAAGCTGATCGCGCAGATAGATGAATACCTG
GATGACACTTTTATGTTGTTCAGCAGTTATGGTATTAATACGCAGGATCTGCAGAAATGGCGGAAGTCAGGTAATCGACT
GTTTCGCTGTTTTGTCAATGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References