Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 4173237..4173916 Replicon chromosome
Accession NZ_CP011612
Organism Citrobacter freundii strain CAV1321

Toxin (Protein)


Gene name ykfI Uniprot ID A0A1B7JLK7
Locus tag AB180_RS22815 Protein ID WP_003031349.1
Coordinates 4173237..4173578 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID A0A1B7JLJ2
Locus tag AB180_RS22820 Protein ID WP_003031347.1
Coordinates 4173599..4173916 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AB180_RS22790 4168982..4169713 + 732 WP_044702485.1 winged helix-turn-helix domain-containing protein -
AB180_RS22795 4169688..4170161 + 474 WP_044702484.1 hypothetical protein -
AB180_RS22800 4170503..4171951 + 1449 WP_044702495.1 EAL domain-containing protein -
AB180_RS22805 4172143..4172607 + 465 WP_044702482.1 isoprenylcysteine carboxylmethyltransferase family protein -
AB180_RS22815 4173237..4173578 - 342 WP_003031349.1 type IV toxin-antitoxin system toxin YkfI Toxin
AB180_RS22820 4173599..4173916 - 318 WP_003031347.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
AB180_RS22825 4173934..4174155 - 222 WP_003031346.1 DUF987 domain-containing protein -
AB180_RS22830 4174164..4174640 - 477 WP_003031345.1 RadC family protein -
AB180_RS22835 4174656..4175117 - 462 WP_003031344.1 antirestriction protein -
AB180_RS28875 4175131..4175322 - 192 Protein_3912 DUF905 family protein -
AB180_RS22840 4175826..4177823 + 1998 WP_016245729.1 choline BCCT transporter BetT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4168982..4188880 19898


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12921.01 Da        Isoelectric Point: 9.6543

>T54687 WP_003031349.1 NZ_CP011612:c4173578-4173237 [Citrobacter freundii]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

>T54687 NZ_CP011612:c4173578-4173237 [Citrobacter freundii]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATAGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGTAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 12094.74 Da        Isoelectric Point: 7.0370

>AT54687 WP_003031347.1 NZ_CP011612:c4173916-4173599 [Citrobacter freundii]
MSKITWGLQRDITPRLGARLVQEGNRLHYLADRASMTGKFSDIEYRKLDETFPHFIRQMESMLTTGELSTHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp

>AT54687 NZ_CP011612:c4173916-4173599 [Citrobacter freundii]
ATGAGCAAAATCACATGGGGTCTGCAGCGTGATATCACGCCACGTCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATGACCGGCAAGTTCAGTGACATCGAATACCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACTGGTGAACTCAGCACCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B7JLK7

Antitoxin

Source ID Structure
AlphaFold DB A0A1B7JLJ2

References