Detailed information of TA system TA054686

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 3733511..3734027
• Replicon: chromosome
• Accession: NZ_CP011612
• Organism: Citrobacter freundii strain CAV1321

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: AB180_RS20850
• Protein ID: WP_044699148.1
• Coordinates: 3733743..3734027 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A0J1MV10
• Locus tag: AB180_RS20845
• Protein ID: WP_003839576.1
• Coordinates: 3733511..3733753 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AB180_RS20825	3728526..3729659	+	1134	WP_016149471.1	amidohydrolase/deacetylase family metallohydrolase	-
AB180_RS20830	3729643..3730761	+	1119	WP_044699152.1	DgaE family pyridoxal phosphate-dependent ammonia lyase	-
AB180_RS20835	3730758..3731498	+	741	WP_044699149.1	KDGP aldolase family protein	-
AB180_RS20840	3731520..3733433	+	1914	WP_003839574.1	BglG family transcription antiterminator	-
AB180_RS20845	3733511..3733753	+	243	WP_003839576.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
AB180_RS20850	3733743..3734027	+	285	WP_044699148.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AB180_RS20855	3734031..3734495	-	465	WP_032937736.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
AB180_RS20860	3734602..3736740	-	2139	WP_003025782.1	anaerobic ribonucleoside-triphosphate reductase	-
AB180_RS20865	3737118..3737702	-	585	WP_003839584.1	fructose PTS transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-82)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-69)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10865.69 Da
• Isoelectric Point: 10.1988

>T54686 WP_044699148.1 NZ_CP011612:3733743-3734027 [Citrobacter freundii]
MTYELEFDPRALKEWHKLGDTVKAQFKKKLADVLLNPRIDSARLNGLPDCYKIKLKSSGYRLVYQVRDEVVTVFVVAVGK
RQHSAVYLDANKRL

Nucleotide

• Length: 285 bp

>T54686 NZ_CP011612:3733743-3734027 [Citrobacter freundii]
ATGACTTATGAACTGGAATTCGACCCGAGAGCCTTAAAAGAGTGGCATAAACTGGGTGATACGGTGAAGGCTCAGTTTAA
GAAGAAGTTGGCCGACGTGTTGTTGAACCCCAGAATTGACTCTGCCCGTTTAAACGGTCTTCCAGATTGCTATAAAATTA
AGCTTAAATCGTCCGGCTATCGTCTGGTGTACCAGGTTCGGGATGAGGTTGTGACCGTGTTTGTTGTCGCGGTCGGCAAG
CGACAACATTCGGCCGTTTATCTCGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8961.34 Da
• Isoelectric Point: 5.0651

>AT54686 WP_003839576.1 NZ_CP011612:3733511-3733753 [Citrobacter freundii]
MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAETGRMPVKTVTVSDSEDTLIQTVRERLASPQKGIKVSLDDL

Nucleotide

• Length: 243 bp

>AT54686 NZ_CP011612:3733511-3733753 [Citrobacter freundii]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCTGTGCGATTACTGTTTCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACCGTTAGCGACA
GCGAAGATACACTGATTCAGACAGTCCGGGAGAGGCTGGCCAGCCCGCAAAAAGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A0J1MV10