54685

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tad-ata/COG5606-COG4679
Location 3477229..3477895 Replicon chromosome
Accession NZ_CP011612
Organism Citrobacter freundii strain CAV1321

Toxin (Protein)


Gene name tad Uniprot ID A0A0D7LIM5
Locus tag AB180_RS19610 Protein ID WP_044702260.1
Coordinates 3477229..3477588 (+) Length 120 a.a.

Antitoxin (Protein)


Gene name ata Uniprot ID A0A0D7LIR0
Locus tag AB180_RS19615 Protein ID WP_003844682.1
Coordinates 3477578..3477895 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AB180_RS19600 3474629..3476260 + 1632 WP_032938230.1 Na/Pi cotransporter family protein -
AB180_RS19605 3476326..3477015 - 690 WP_003841421.1 dipeptidase PepE -
AB180_RS19610 3477229..3477588 + 360 WP_044702260.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AB180_RS19615 3477578..3477895 + 318 WP_003844682.1 helix-turn-helix domain-containing protein Antitoxin
AB180_RS19620 3477905..3478183 - 279 WP_044702262.1 putative addiction module antidote protein -
AB180_RS28830 3478587..3478748 - 162 WP_003841416.1 phage protein -
AB180_RS19630 3478890..3479246 + 357 WP_032948294.1 hypothetical protein -
AB180_RS19635 3479298..3480110 - 813 WP_044702264.1 shikimate 5-dehydrogenase -
AB180_RS19640 3480112..3481335 - 1224 WP_044702265.1 L-sorbose 1-phosphate reductase -
AB180_RS19645 3481404..3482228 - 825 WP_003031641.1 PTS system mannose/fructose/sorbose family transporter subunit IID -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(14-106)

Antitoxin

HigA2-like, helix-turn-helix domain

(12-90)


Sequences

Toxin        


Download         Length: 120 a.a.        Molecular weight: 13437.49 Da        Isoelectric Point: 10.2555

>T54685 WP_044702260.1 NZ_CP011612:3477229-3477588 [Citrobacter freundii]
MTKPLYWVGQARKDLIAMPEHVRDTFGFALWLAQQGKQHSQTKPLKGFGGAGVLEVVEDYHGNAWRAVYTIQLKNAVYVL
HVFQKKSVSGKATPKPEIDLIYQRLKAAQRHAQESGYVT

Download         Length: 360 bp

>T54685 NZ_CP011612:3477229-3477588 [Citrobacter freundii]
ATGACGAAACCACTTTACTGGGTTGGGCAGGCCAGAAAAGACCTGATTGCAATGCCAGAACACGTTCGGGATACGTTTGG
GTTTGCTCTTTGGCTTGCGCAACAGGGTAAACAGCATTCACAGACTAAACCATTAAAAGGATTTGGTGGAGCGGGGGTTC
TGGAAGTCGTTGAGGATTATCACGGCAACGCCTGGCGGGCGGTTTACACCATCCAGTTGAAAAATGCGGTTTATGTTCTC
CATGTTTTTCAGAAGAAGTCCGTTTCCGGGAAAGCAACCCCGAAACCTGAAATTGACCTGATTTATCAGCGGCTCAAGGC
TGCACAGCGCCATGCGCAGGAGAGTGGATATGTCACATGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11466.35 Da        Isoelectric Point: 9.0005

>AT54685 WP_003844682.1 NZ_CP011612:3477578-3477895 [Citrobacter freundii]
MSHDVETSSGNVYADLGAYDAEQMQLKAQLAMVIGEILQRKGLTQQQAAQLLGMTQPKLSNLLRGQFRGISEAKMLECLV
RLGRDVEIVVGKEGNMSGKLKVKFG

Download         Length: 318 bp

>AT54685 NZ_CP011612:3477578-3477895 [Citrobacter freundii]
ATGTCACATGATGTCGAAACCAGTAGCGGTAATGTTTACGCCGATTTAGGTGCGTATGACGCAGAACAGATGCAGTTGAA
AGCGCAGCTGGCAATGGTGATTGGTGAGATTTTGCAACGCAAAGGGTTAACGCAGCAGCAGGCCGCGCAGTTACTGGGAA
TGACACAGCCTAAACTTTCCAATCTGCTACGCGGCCAGTTTCGCGGTATCAGTGAAGCAAAGATGCTCGAATGCCTGGTG
AGGCTGGGCCGTGATGTAGAGATTGTTGTCGGGAAAGAGGGCAATATGTCAGGAAAGCTGAAAGTGAAATTTGGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0D7LIM5

Antitoxin

Source ID Structure
AlphaFold DB A0A0D7LIR0

References