54684

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 3325339..3325955 Replicon chromosome
Accession NZ_CP011612
Organism Citrobacter freundii strain CAV1321

Toxin (Protein)


Gene name vapC Uniprot ID A0A0J1MQ96
Locus tag AB180_RS18915 Protein ID WP_003028682.1
Coordinates 3325581..3325955 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A6B5NTK4
Locus tag AB180_RS18910 Protein ID WP_043018956.1
Coordinates 3325339..3325581 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AB180_RS18880 3321189..3321788 + 600 WP_016151258.1 glucose-1-phosphatase -
AB180_RS18885 3321782..3322654 + 873 WP_003028669.1 virulence factor BrkB family protein -
AB180_RS18890 3322651..3323088 + 438 WP_003028671.1 D-tyrosyl-tRNA(Tyr) deacylase -
AB180_RS18895 3323133..3324074 + 942 WP_003825286.1 fatty acid biosynthesis protein FabY -
AB180_RS18905 3324225..3325133 - 909 WP_044701816.1 alpha/beta hydrolase -
AB180_RS18910 3325339..3325581 + 243 WP_043018956.1 ribbon-helix-helix domain-containing protein Antitoxin
AB180_RS18915 3325581..3325955 + 375 WP_003028682.1 type II toxin-antitoxin system VapC family toxin Toxin
AB180_RS18920 3325992..3326921 - 930 WP_003028685.1 formate dehydrogenase accessory protein FdhE -
AB180_RS18925 3326918..3327553 - 636 WP_003028686.1 formate dehydrogenase cytochrome b556 subunit -
AB180_RS18930 3327550..3328452 - 903 WP_003028691.1 formate dehydrogenase O subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(13-47)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13674.89 Da        Isoelectric Point: 9.5622

>T54684 WP_003028682.1 NZ_CP011612:3325581-3325955 [Citrobacter freundii]
MVKGSALFDTNILIDLFSGRNEAKLAIETWPPQNAISLITWMEVMVGAKKYHQEARTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTKDFAGLSGVVTPYTL

Download         Length: 375 bp

>T54684 NZ_CP011612:3325581-3325955 [Citrobacter freundii]
ATGGTCAAAGGATCCGCGCTTTTTGATACCAACATCCTCATCGATCTGTTTAGTGGGAGAAATGAAGCAAAACTAGCGAT
AGAAACATGGCCGCCGCAGAATGCAATCAGCCTCATTACATGGATGGAGGTGATGGTGGGTGCAAAAAAATACCATCAGG
AAGCCCGTACTCGTGTTGCGCTAGGTGCTTTTAACGTGATCGGCGTTTCACAGGATATTGCTGAACGAAGCGTGAGTATC
AGACAGGAATATGGGATGAAATTGCCGGATGCCATTATTTTGGCAACAGCACAGATTCATCGTCTTACGCTGGTTACGCG
AAATACTAAGGATTTTGCAGGCCTATCCGGTGTGGTCACACCGTATACATTATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9451.75 Da        Isoelectric Point: 4.3437

>AT54684 WP_043018956.1 NZ_CP011612:3325339-3325581 [Citrobacter freundii]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp

>AT54684 NZ_CP011612:3325339-3325581 [Citrobacter freundii]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGATGAAGTGATTAAACGTCTTGATGATCTCAAGGT
GCAGCGCAACCTGCCGCGAGCAGAACTTTTACGGGAGGCCGTTGAACAGTATCTTGAGAGACAGGATCAGGCTGAAACCA
CAATTTCACGTGCTCTTGGGCTGTGGCAAGGCTGTGAGGAAGATGGTGTTGAATACCAGCGTAAACTCCGTGAGGAATGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1MQ96

Antitoxin

Source ID Structure
AlphaFold DB A0A6B5NTK4

References