Detailed information of TA system TA054680

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 2439439..2440103
• Replicon: chromosome
• Accession: NZ_CP011612
• Organism: Citrobacter freundii strain CAV1321

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: AB180_RS14630
• Protein ID: WP_047715922.1
• Coordinates: 2439439..2439780 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: A0A1D3BNC1
• Locus tag: AB180_RS14635
• Protein ID: WP_032666156.1
• Coordinates: 2439801..2440103 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AB180_RS14610	2435482..2436531	+	1050	WP_044700921.1	YncE family protein	-
AB180_RS14615	2436542..2438539	+	1998	WP_003846276.1	TonB-dependent receptor	-
AB180_RS14620	2438570..2439076	-	507	WP_044700923.1	G/U mismatch-specific DNA glycosylase	-
AB180_RS14630	2439439..2439780	-	342	WP_047715922.1	toxin	Toxin
AB180_RS14635	2439801..2440103	-	303	WP_032666156.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AB180_RS14640	2440137..2440358	-	222	WP_047715924.1	DUF987 domain-containing protein	-
AB180_RS14645	2440367..2440843	-	477	WP_047715925.1	RadC family protein	-
AB180_RS14650	2440859..2441317	-	459	WP_032666161.1	antirestriction protein	-
AB180_RS14655	2441420..2441659	-	240	WP_032666162.1	DUF905 domain-containing protein	-
AB180_RS14660	2441736..2442203	-	468	WP_032666163.1	hypothetical protein	-
AB180_RS14665	2442227..2442670	-	444	WP_032666164.1	hypothetical protein	-
AB180_RS14670	2442670..2442906	-	237	WP_001144029.1	hypothetical protein	-
AB180_RS14675	2442947..2443648	-	702	WP_032666165.1	WYL domain-containing protein	-
AB180_RS14680	2443865..2444686	-	822	WP_032666166.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-99)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13019.14 Da
• Isoelectric Point: 10.2220

>T54680 WP_047715922.1 NZ_CP011612:c2439780-2439439 [Citrobacter freundii]
MKTLPATTQRAVKPCLSPVVVWQMLLTRLLEQHYGLTLNDTPFGDERVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQVTGLLRQSRKNSVR

Nucleotide

• Length: 342 bp

>T54680 NZ_CP011612:c2439780-2439439 [Citrobacter freundii]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGTGAAGCCCTGCTTGTCACCCGTAGTTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACACTGAACGATACGCCGTTCGGTGATGAAAGGGTTATTAAGGAACATATCG
ATGCTGGTATCACTCTGGCTGATGCCGTGAATTTTCTGGTGGAAAAATACGAACTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCCCCTTACCTTCGGGCTGTCGATATTCTGCGAGCGCGGCAGGTAACTGGCTTGTTGCGGCAAAG
CCGTAAAAATTCGGTACGGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11186.65 Da
• Isoelectric Point: 6.3129

>AT54680 WP_032666156.1 NZ_CP011612:c2440103-2439801 [Citrobacter freundii]
MGPAAEYTPRLGARLVQEGNRLHYLADRASITGMFSDAECRKLDDTFPHFIRQMESMLATGELSPQHAHCVTLYHNGFTC
EADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 303 bp

>AT54680 NZ_CP011612:c2440103-2439801 [Citrobacter freundii]
CTGGGGCCTGCAGCGGAATACACGCCGCGCCTAGGAGCTCGTCTGGTGCAGGAGGGCAACCGGCTGCATTATCTGGCTGA
CCGGGCCAGCATCACCGGTATGTTCAGTGACGCCGAATGTCGGAAGCTGGATGACACATTCCCGCATTTTATCCGCCAGA
TGGAATCGATGCTGGCCACCGGTGAACTCAGCCCTCAACATGCCCACTGCGTCACCCTGTACCACAACGGTTTCACCTGC
GAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A1D3BNC1