54680

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 2439439..2440103 Replicon chromosome
Accession NZ_CP011612
Organism Citrobacter freundii strain CAV1321

Toxin (Protein)


Gene name ykfI Uniprot ID -
Locus tag AB180_RS14630 Protein ID WP_047715922.1
Coordinates 2439439..2439780 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID A0A1D3BNC1
Locus tag AB180_RS14635 Protein ID WP_032666156.1
Coordinates 2439801..2440103 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AB180_RS14610 2435482..2436531 + 1050 WP_044700921.1 YncE family protein -
AB180_RS14615 2436542..2438539 + 1998 WP_003846276.1 TonB-dependent receptor -
AB180_RS14620 2438570..2439076 - 507 WP_044700923.1 G/U mismatch-specific DNA glycosylase -
AB180_RS14630 2439439..2439780 - 342 WP_047715922.1 toxin Toxin
AB180_RS14635 2439801..2440103 - 303 WP_032666156.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
AB180_RS14640 2440137..2440358 - 222 WP_047715924.1 DUF987 domain-containing protein -
AB180_RS14645 2440367..2440843 - 477 WP_047715925.1 RadC family protein -
AB180_RS14650 2440859..2441317 - 459 WP_032666161.1 antirestriction protein -
AB180_RS14655 2441420..2441659 - 240 WP_032666162.1 DUF905 domain-containing protein -
AB180_RS14660 2441736..2442203 - 468 WP_032666163.1 hypothetical protein -
AB180_RS14665 2442227..2442670 - 444 WP_032666164.1 hypothetical protein -
AB180_RS14670 2442670..2442906 - 237 WP_001144029.1 hypothetical protein -
AB180_RS14675 2442947..2443648 - 702 WP_032666165.1 WYL domain-containing protein -
AB180_RS14680 2443865..2444686 - 822 WP_032666166.1 DUF945 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(6-99)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13019.14 Da        Isoelectric Point: 10.2220

>T54680 WP_047715922.1 NZ_CP011612:c2439780-2439439 [Citrobacter freundii]
MKTLPATTQRAVKPCLSPVVVWQMLLTRLLEQHYGLTLNDTPFGDERVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQVTGLLRQSRKNSVR

Download         Length: 342 bp

>T54680 NZ_CP011612:c2439780-2439439 [Citrobacter freundii]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGTGAAGCCCTGCTTGTCACCCGTAGTTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACACTGAACGATACGCCGTTCGGTGATGAAAGGGTTATTAAGGAACATATCG
ATGCTGGTATCACTCTGGCTGATGCCGTGAATTTTCTGGTGGAAAAATACGAACTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCCCCTTACCTTCGGGCTGTCGATATTCTGCGAGCGCGGCAGGTAACTGGCTTGTTGCGGCAAAG
CCGTAAAAATTCGGTACGGTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11186.65 Da        Isoelectric Point: 6.3129

>AT54680 WP_032666156.1 NZ_CP011612:c2440103-2439801 [Citrobacter freundii]
MGPAAEYTPRLGARLVQEGNRLHYLADRASITGMFSDAECRKLDDTFPHFIRQMESMLATGELSPQHAHCVTLYHNGFTC
EADTLGSCGYVYIAIYPTQR

Download         Length: 303 bp

>AT54680 NZ_CP011612:c2440103-2439801 [Citrobacter freundii]
CTGGGGCCTGCAGCGGAATACACGCCGCGCCTAGGAGCTCGTCTGGTGCAGGAGGGCAACCGGCTGCATTATCTGGCTGA
CCGGGCCAGCATCACCGGTATGTTCAGTGACGCCGAATGTCGGAAGCTGGATGACACATTCCCGCATTTTATCCGCCAGA
TGGAATCGATGCTGGCCACCGGTGAACTCAGCCCTCAACATGCCCACTGCGTCACCCTGTACCACAACGGTTTCACCTGC
GAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A1D3BNC1

References