Detailed information of TA system TA054670

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 206721..207622
• Replicon: plasmid pKPC_CAV1321-244
• Accession: NZ_CP011611
• Organism: Citrobacter freundii strain CAV1321

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A837LJP8
• Locus tag: AB180_RS02755
• Protein ID: WP_007372300.1
• Coordinates: 206721..207179 (-)
• Length: 153 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: AB180_RS02760
• Protein ID: WP_008786588.1
• Coordinates: 207176..207622 (-)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AB180_RS02710	202447..202689	+	243	WP_007372291.1	hypothetical protein	-
AB180_RS02715	202700..203098	+	399	WP_007372292.1	hypothetical protein	-
AB180_RS02720	203182..203508	+	327	WP_007372293.1	hypothetical protein	-
AB180_RS02725	203871..204209	-	339	WP_007372294.1	hypothetical protein	-
AB180_RS02730	204447..204890	+	444	WP_007372295.1	hypothetical protein	-
AB180_RS02735	205009..205350	+	342	WP_007372296.1	hypothetical protein	-
AB180_RS02740	205509..206063	+	555	WP_047715717.1	hypothetical protein	-
AB180_RS02745	206113..206379	+	267	WP_007372298.1	hypothetical protein	-
AB180_RS02750	206447..206677	-	231	WP_007372299.1	hypothetical protein	-
AB180_RS02755	206721..207179	-	459	WP_007372300.1	RES family NAD+ phosphorylase	Toxin
AB180_RS02760	207176..207622	-	447	WP_008786588.1	DUF2384 domain-containing protein	Antitoxin
AB180_RS02765	207771..208088	+	318	WP_007372302.1	hypothetical protein	-
AB180_RS02770	208130..208972	+	843	WP_007372303.1	hypothetical protein	-
AB180_RS02775	208944..209222	+	279	WP_007372304.1	hypothetical protein	-
AB180_RS02780	209282..209725	+	444	WP_007372305.1	hypothetical protein	-
AB180_RS28590	210012..210176	+	165	WP_007372306.1	hypothetical protein	-
AB180_RS02785	210744..211586	+	843	WP_007372307.1	HNH endonuclease	-
AB180_RS02790	212008..212259	+	252	WP_024196080.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(3)-IIa / aac(6')-Ib-cr / blaOXA-1 / catB3 / ARR-3 / qacE / sul1 / blaTEM-1B / blaKPC-2 / tet(D) / catA2 / sul2	-	1..243709	243709

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (95-145)

Sequences

Toxin

Protein

• Length: 153 a.a.
• Molecular weight: 16975.40 Da
• Isoelectric Point: 4.5730

>T54670 WP_007372300.1 NZ_CP011611:c207179-206721 [Citrobacter freundii]
VILYRLTKTRYLMTAWSGLGAKEAGGRWNSVGTAMVYLSETASLTMLETLVHIHAPQLLDDFTLLSLDVPDDQIQTFDMS
RLPDNWASEDAPAELALYGDNWAESGSSIALRIPSALSPVEFNYLLNPAHPELFDLMATVKKIPFRFDSRLK

Nucleotide

• Length: 459 bp

>T54670 NZ_CP011611:c207179-206721 [Citrobacter freundii]
GTGATCCTTTACCGACTGACCAAGACCCGTTATCTGATGACTGCGTGGTCTGGTCTGGGTGCGAAAGAAGCGGGGGGCCG
CTGGAACAGCGTAGGCACTGCAATGGTCTACCTGTCTGAAACGGCCAGCCTTACCATGCTTGAAACGCTGGTACACATTC
ACGCGCCGCAGTTACTGGACGACTTTACACTGCTGAGTCTTGATGTACCGGATGATCAGATCCAGACCTTCGACATGAGC
CGTCTCCCTGATAACTGGGCGAGTGAGGATGCTCCTGCTGAGCTGGCGCTCTACGGTGATAACTGGGCTGAAAGCGGTTC
TTCTATCGCGCTGCGTATTCCAAGTGCGTTGTCTCCGGTTGAATTTAACTACTTGCTGAATCCGGCACACCCTGAACTCT
TTGACCTGATGGCTACGGTTAAGAAAATTCCGTTCCGGTTTGATTCCCGCCTGAAATAA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16689.02 Da
• Isoelectric Point: 6.9812

>AT54670 WP_008786588.1 NZ_CP011611:c207622-207176 [Citrobacter freundii]
MTMKVFSPDVTKTNQHCLWQVAGLDNDGIALMDKINHGLDGTVAKHISEWANITPSELRKMSGIPNTTFNRSIKDRFTAD
QSERLVRIIRVIERAVELFEGDKEAAHKWLNEANRGLSWKSPAELVSSETGALEVMRLITRIEHGVYS

Nucleotide

• Length: 447 bp

>AT54670 NZ_CP011611:c207622-207176 [Citrobacter freundii]
ATGACGATGAAAGTTTTTTCTCCAGACGTTACCAAAACGAATCAGCACTGCTTATGGCAGGTTGCTGGGCTGGATAATGA
CGGTATCGCGCTGATGGATAAAATCAATCACGGTCTCGATGGCACAGTAGCTAAGCACATTTCTGAATGGGCTAACATCA
CGCCGTCTGAGCTGCGCAAAATGTCGGGTATACCTAATACGACGTTTAATCGTAGCATCAAAGACCGTTTCACTGCCGAT
CAGAGCGAACGTCTGGTGCGCATTATCCGCGTAATTGAACGCGCTGTAGAGCTGTTTGAAGGTGACAAAGAAGCGGCGCA
CAAATGGCTGAACGAAGCGAACCGTGGTCTGAGCTGGAAATCACCGGCAGAGCTTGTTTCTTCTGAGACAGGTGCGCTTG
AAGTGATGCGCCTGATTACGCGTATTGAGCACGGGGTATACTCGTGA

Structures

Toxin

• AlphaFold DB: A0A837LJP8