Detailed information of TA system TA054669

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 199422..199972
• Replicon: plasmid pKPC_CAV1321-244
• Accession: NZ_CP011611
• Organism: Citrobacter freundii strain CAV1321

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: S1EZ93
• Locus tag: AB180_RS02690
• Protein ID: WP_007372286.1
• Coordinates: 199664..199972 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1FMC3
• Locus tag: AB180_RS02685
• Protein ID: WP_007372285.1
• Coordinates: 199422..199661 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AB180_RS02645	194429..194824	+	396	WP_024196081.1	hypothetical protein	-
AB180_RS02650	195006..195197	+	192	WP_016241560.1	hypothetical protein	-
AB180_RS02655	195328..196023	+	696	WP_007372278.1	hypothetical protein	-
AB180_RS26780	196046..196243	+	198	WP_032155220.1	Lar family restriction alleviation protein	-
AB180_RS02660	196296..196754	+	459	WP_008786599.1	hypothetical protein	-
AB180_RS28580	196902..197042	+	141	WP_007372281.1	hypothetical protein	-
AB180_RS02665	197058..197690	+	633	WP_007372282.1	hypothetical protein	-
AB180_RS02675	198151..198822	+	672	WP_007372283.1	hypothetical protein	-
AB180_RS28940	198819..199283	+	465	WP_008786596.1	hypothetical protein	-
AB180_RS02685	199422..199661	+	240	WP_007372285.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
AB180_RS02690	199664..199972	+	309	WP_007372286.1	CcdB family protein	Toxin
AB180_RS28585	199993..200151	+	159	WP_016241558.1	hypothetical protein	-
AB180_RS02695	200306..200860	+	555	WP_007372288.1	hypothetical protein	-
AB180_RS02700	200930..201547	+	618	WP_007372289.1	hypothetical protein	-
AB180_RS02705	201630..202100	+	471	WP_007372290.1	hypothetical protein	-
AB180_RS02710	202447..202689	+	243	WP_007372291.1	hypothetical protein	-
AB180_RS02715	202700..203098	+	399	WP_007372292.1	hypothetical protein	-
AB180_RS02720	203182..203508	+	327	WP_007372293.1	hypothetical protein	-
AB180_RS02725	203871..204209	-	339	WP_007372294.1	hypothetical protein	-
AB180_RS02730	204447..204890	+	444	WP_007372295.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(3)-IIa / aac(6')-Ib-cr / blaOXA-1 / catB3 / ARR-3 / qacE / sul1 / blaTEM-1B / blaKPC-2 / tet(D) / catA2 / sul2	-	1..243709	243709

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-101)

Antitoxin

• Post-segregation antitoxin CcdA (9-79)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11351.17 Da
• Isoelectric Point: 8.5044

>T54669 WP_007372286.1 NZ_CP011611:199664-199972 [Citrobacter freundii]
MQYTVYRNPGNSQAYPYLLDIQSDIIGELNTRLVIPLHRLKKGASAPVARLTPVIQVEGNDVILMTHEMASVRVKQLGQA
VMDASPFRHTIKSAVDFLLDGF

Nucleotide

• Length: 309 bp

>T54669 NZ_CP011611:199664-199972 [Citrobacter freundii]
ATGCAATATACCGTTTACCGCAATCCCGGCAACAGCCAGGCATACCCTTACCTGCTGGATATACAGAGCGACATCATTGG
CGAGTTAAATACCCGCCTGGTGATCCCCCTTCATCGGCTTAAAAAAGGAGCCTCGGCCCCGGTTGCGCGATTAACTCCGG
TCATACAGGTGGAAGGGAATGACGTTATCCTGATGACGCATGAAATGGCTTCAGTCCGCGTGAAGCAGCTCGGTCAGGCG
GTCATGGACGCATCTCCGTTCCGGCACACTATCAAAAGTGCGGTGGACTTCCTGCTGGATGGTTTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8784.97 Da
• Isoelectric Point: 8.6387

>AT54669 WP_007372285.1 NZ_CP011611:199422-199661 [Citrobacter freundii]
MQATAARQKKTVSVTLEPMLVQQAKDAGINLSATLAEALQSKLKASAAEEWKRQNRAGIQELNRITEEHGLLSDEYRTF

Nucleotide

• Length: 240 bp

>AT54669 NZ_CP011611:199422-199661 [Citrobacter freundii]
ATGCAGGCTACAGCTGCTCGCCAGAAAAAAACCGTCAGTGTCACCCTTGAGCCAATGCTCGTACAGCAGGCAAAAGACGC
TGGGATTAACTTGTCCGCCACGCTGGCAGAAGCGCTTCAGAGCAAACTGAAGGCCAGTGCAGCGGAAGAATGGAAACGAC
AGAATCGTGCTGGTATTCAGGAGTTGAACCGCATCACTGAAGAGCATGGGTTACTGTCTGACGAATACAGGACGTTCTGA

Structures

Toxin

• AlphaFold DB: S1EZ93

Antitoxin

• AlphaFold DB: S1FMC3