Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/ElaA-DUF1778
Location 28919..29722 Replicon plasmid pCAV1321-135
Accession NZ_CP011610
Organism Citrobacter freundii strain CAV1321

Toxin (Protein)


Gene name KacT Uniprot ID A0A7W3E3C9
Locus tag AB180_RS00960 Protein ID WP_022652313.1
Coordinates 28919..29449 (-) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID W8E6Q5
Locus tag AB180_RS00965 Protein ID WP_022652312.1
Coordinates 29453..29722 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AB180_RS00935 24805..25152 - 348 WP_000609174.1 IS66 family insertion sequence element accessory protein TnpB -
AB180_RS00940 25149..25532 - 384 WP_001201739.1 IS66 family insertion sequence hypothetical protein -
AB180_RS00945 25641..26792 - 1152 WP_022652315.1 IS30-like element IS30 family transposase -
AB180_RS00950 26857..27753 + 897 Protein_29 IS3 family transposase -
AB180_RS00955 27894..28787 - 894 WP_086538014.1 IS5 family transposase -
AB180_RS00960 28919..29449 - 531 WP_022652313.1 GNAT family N-acetyltransferase Toxin
AB180_RS00965 29453..29722 - 270 WP_022652312.1 DUF1778 domain-containing protein Antitoxin
AB180_RS00970 30578..31567 + 990 WP_022652311.1 RepB family plasmid replication initiator protein -
AB180_RS00975 31893..32633 - 741 WP_022652310.1 site-specific integrase -
AB180_RS00980 33110..33417 + 308 Protein_35 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(D) - 1..135117 135117
- inside IScluster/Tn - - 21350..34731 13381


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(92-156)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 177 a.a.        Molecular weight: 20349.26 Da        Isoelectric Point: 6.7496

>T54667 WP_022652313.1 NZ_CP011610:c29449-28919 [Citrobacter freundii]
MEGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYVLVTRENKPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCNSL
FYPTKSIEELFEVNDE

Download         Length: 531 bp

>T54667 NZ_CP011610:c29449-28919 [Citrobacter freundii]
ATGGAGGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATGAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTTACTGACCATCTGAAACGGCAGCATAACAGTAAGATCTTACGTGGTTATGTTCTCGTGACGAGAGAGA
ATAAACCGCGTGTGATGGGGTACTACACGCTATCAGGAAGTTGCTTTGAGAAAATACTTCTACCCAGCAAAACACAACAA
AAGCGTGTACCCTACAAGAATGTGCCGAGCGTGACTTTGGGCCGTCTGGCAATTGATAAAAGCATTCACCACCAGGGATA
TGGAGAAACACTTGTTACCCATGCGATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGTATACACGGAATGTTCGTGG
AAGCGCTGAACGACAACGCCAAAAAATTCTACCTTCGACTGGGTTTCATTCAGTTAAAGGAAGAAAACTGTAACTCGCTC
TTTTACCCGACAAAATCTATTGAAGAATTGTTCGAAGTAAATGATGAATAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10269.70 Da        Isoelectric Point: 5.7252

>AT54667 WP_022652312.1 NZ_CP011610:c29722-29453 [Citrobacter freundii]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAIINPPAPNDRLKR
AANRLRELE

Download         Length: 270 bp

>AT54667 NZ_CP011610:c29722-29453 [Citrobacter freundii]
ATGTCAGCACTGAAAAAGCAGCGCATTGACCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCAATTTCGCAGTTCATGGTCAGTACCGCCTCAGAACGCGCTGCAGAAGTTATTGACCAGCACCGTCGTC
TGCTCCTCAATGAGGAGTCTTGGAACCTGGTTATGGATGCAATCATTAATCCGCCAGCACCGAATGACCGGCTGAAACGC
GCCGCTAACCGTCTCCGGGAACTGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W3E3C9

Antitoxin

Source ID Structure
AlphaFold DB A0A2Z3XDS5

References