Detailed information of TA system TA054654

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 2147044..2147663
• Replicon: chromosome
• Accession: NZ_CP011597
• Organism: Klebsiella oxytoca strain CAV1099

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: H3N9D8
• Locus tag: AB184_RS12305
• Protein ID: WP_004099646.1
• Coordinates: 2147445..2147663 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: AB184_RS12300
• Protein ID: WP_004099648.1
• Coordinates: 2147044..2147418 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AB184_RS12290	2142200..2143393	+	1194	WP_004111040.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
AB184_RS12295	2143416..2146562	+	3147	WP_004099650.1	multidrug efflux RND transporter permease subunit	-
AB184_RS12300	2147044..2147418	+	375	WP_004099648.1	Hha toxicity modulator TomB	Antitoxin
AB184_RS12305	2147445..2147663	+	219	WP_004099646.1	hemolysin expression modulator Hha	Toxin
AB184_RS12310	2147824..2148390	+	567	WP_004099644.1	maltose O-acetyltransferase	-
AB184_RS12315	2148527..2148997	+	471	WP_004111038.1	YlaC family protein	-
AB184_RS12320	2148972..2150426	-	1455	WP_024273853.1	PLP-dependent aminotransferase family protein	-
AB184_RS12325	2150529..2151227	+	699	WP_004099639.1	GNAT family N-acetyltransferase	-
AB184_RS32160	2151224..2151364	-	141	WP_003859006.1	type B 50S ribosomal protein L36	-
AB184_RS12330	2151364..2151627	-	264	WP_004099638.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8640.05 Da
• Isoelectric Point: 8.9008

>T54654 WP_004099646.1 NZ_CP011597:2147445-2147663 [Klebsiella oxytoca]
MSDKTLTKIDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWKFIR

Nucleotide

• Length: 219 bp

>T54654 NZ_CP011597:2147445-2147663 [Klebsiella oxytoca]
ATGTCTGATAAAACATTAACCAAAATAGACTATTTGATGCGCTTACGACGTTGTCAGACAATTGATACGCTGGAGCGCGT
AATTGAAAAAAATAAATATGAACTTTCTGATAATGAACTGGCGGTATTTTACTCAGCAGCCGATCATCGTCTTGCTGAAC
TGACGATGAATAAACTGTATGACAAGATCCCGCCATCGGTCTGGAAATTCATCCGCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14350.09 Da
• Isoelectric Point: 4.8989

>AT54654 WP_004099648.1 NZ_CP011597:2147044-2147418 [Klebsiella oxytoca]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDNKLVTQLDEYL
DDTFVLFSNYGINTADLQKWRKSGNRLFRCFVNASRENPASLSC

Nucleotide

• Length: 375 bp

>AT54654 NZ_CP011597:2147044-2147418 [Klebsiella oxytoca]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTTAATGACCCAACCTCTGCTGTCAATCTTCAGCTTAATGAATTGATCGAGC
ACATTGCCGCGTTCGCACTTAATTATAAAATTAAGTATGCTGAGGATAATAAGCTGGTGACTCAGCTTGACGAATATCTG
GATGACACTTTCGTGTTGTTCAGCAACTACGGCATTAACACTGCAGATCTGCAGAAATGGCGTAAATCAGGCAACAGGTT
ATTCCGCTGCTTTGTGAACGCCAGTCGGGAAAACCCGGCCAGTCTTTCTTGTTAA

Structures

Toxin

• AlphaFold DB: A0A0H3H713