Detailed information of TA system TA054653

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 67331..68067
• Replicon: plasmid pCAV1099-114
• Accession: NZ_CP011596
• Organism: Klebsiella oxytoca strain CAV1099

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: AB184_RS02045
• Protein ID: WP_003026803.1
• Coordinates: 67585..68067 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: AB184_RS02040
• Protein ID: WP_003026799.1
• Coordinates: 67331..67597 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AB184_RS02010	62798..63766	-	969	WP_077257929.1	IS5-like element IS903B family transposase	-
AB184_RS02015	63793..64023	+	231	Protein_68	oxidoreductase	-
AB184_RS31725	64254..64445	+	192	Protein_69	IS1 family transposase	-
AB184_RS02020	64497..65465	-	969	WP_176678774.1	IS5 family transposase	-
AB184_RS02025	65887..66378	+	492	WP_004206662.1	hypothetical protein	-
AB184_RS02030	66439..66642	+	204	WP_004213596.1	hemolysin expression modulator Hha	-
AB184_RS02035	66656..66886	+	231	WP_004206660.1	hypothetical protein	-
AB184_RS02040	67331..67597	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
AB184_RS02045	67585..68067	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
AB184_RS02050	68268..69671	+	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
AB184_RS02055	69700..70332	-	633	WP_001567369.1	hypothetical protein	-
AB184_RS02060	70407..70778	+	372	Protein_78	IS5/IS1182 family transposase	-
AB184_RS02065	70810..71778	+	969	WP_013815099.1	IS5-like element IS903B family transposase	-
AB184_RS02070	71939..72754	+	816	WP_012695470.1	aminoglycoside O-phosphotransferase APH(3')-Ia	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul1 / aadA2 / aph(3')-Ia / mcr-9 / aph(6)-Id / aph(3'')-Ib / dfrA19 / qnrB2	pla	1..113992	113992
	-	inside	IScluster/Tn	aph(3')-Ia	-	62798..78410	15612

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T54653 WP_003026803.1 NZ_CP011596:67585-68067 [Klebsiella oxytoca]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T54653 NZ_CP011596:67585-68067 [Klebsiella oxytoca]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT54653 WP_003026799.1 NZ_CP011596:67331-67597 [Klebsiella oxytoca]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT54653 NZ_CP011596:67331-67597 [Klebsiella oxytoca]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66