Detailed information of TA system TA054650

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 3149660..3150276
• Replicon: chromosome
• Accession: NZ_CP011591
• Organism: Enterobacter asburiae strain CAV1043

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AB190_RS16535
• Protein ID: WP_080298642.1
• Coordinates: 3149905..3150276 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A1Z3N2D4
• Locus tag: AB190_RS16530
• Protein ID: WP_047743309.1
• Coordinates: 3149660..3149902 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AB190_RS16505	3145637..3146236	+	600	WP_047743306.1	glucose-1-phosphatase	-
AB190_RS16510	3146230..3147111	+	882	WP_045355564.1	virulence factor BrkB family protein	-
AB190_RS16515	3147108..3147545	+	438	WP_008501836.1	D-tyrosyl-tRNA(Tyr) deacylase	-
AB190_RS16520	3147590..3148531	+	942	WP_023309755.1	fatty acid biosynthesis protein FabY	-
AB190_RS16525	3148540..3149460	-	921	WP_047743308.1	alpha/beta hydrolase	-
AB190_RS16530	3149660..3149902	+	243	WP_047743309.1	ribbon-helix-helix protein, CopG family	Antitoxin
AB190_RS16535	3149905..3150276	+	372	WP_080298642.1	type II toxin-antitoxin system VapC family toxin	Toxin
AB190_RS16540	3150316..3151245	-	930	WP_023309751.1	formate dehydrogenase accessory protein FdhE	-
AB190_RS16545	3151242..3151877	-	636	WP_023309750.1	formate dehydrogenase cytochrome b556 subunit	-
AB190_RS16550	3151874..3152776	-	903	WP_008501831.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-109)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13688.85 Da
• Isoelectric Point: 6.9789

>T54650 WP_080298642.1 NZ_CP011591:3149905-3150276 [Enterobacter asburiae]
MEHMAVFDTNILIDLFNNRVEAADAIDRTASHRAISLITWMEVMVGARKHGHEAKTATVMGVFEIIDITRDIAERSVILR
EKHGMKLPDAIILATAQSRNCPLISRNTKDFSGITGVVSPYQL

Nucleotide

• Length: 372 bp

>T54650 NZ_CP011591:3149905-3150276 [Enterobacter asburiae]
ATGGAGCACATGGCGGTGTTCGATACCAATATACTCATTGATCTATTTAATAATCGCGTTGAGGCTGCGGATGCAATTGA
TCGCACAGCTTCGCATCGAGCCATTAGCCTGATCACATGGATGGAAGTCATGGTGGGTGCCCGCAAACATGGTCACGAAG
CGAAAACTGCGACAGTGATGGGCGTATTTGAGATTATTGATATCACCCGAGATATCGCTGAAAGAAGTGTAATTCTGCGC
GAGAAGCATGGAATGAAACTGCCTGATGCTATCATTCTGGCCACAGCTCAGAGCAGAAATTGTCCGTTGATTTCTCGAAA
CACGAAAGACTTTTCGGGCATTACTGGAGTCGTTTCACCTTACCAACTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9443.59 Da
• Isoelectric Point: 4.2917

>AT54650 WP_047743309.1 NZ_CP011591:3149660-3149902 [Enterobacter asburiae]
MSLSADLDMGRILIDLSDDVIQRLENLKQLRNRPRAELLREAIEQYLDQQSSSVIRDALGLWGNQQEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT54650 NZ_CP011591:3149660-3149902 [Enterobacter asburiae]
ATGAGTCTTTCCGCGGATCTTGATATGGGCAGAATTCTGATCGATTTGTCTGATGACGTAATTCAACGACTGGAAAATCT
GAAGCAGCTGCGTAACCGGCCTCGTGCCGAATTGCTTCGGGAAGCCATTGAGCAATATCTCGACCAGCAGAGTTCATCGG
TTATCCGCGATGCGCTTGGCTTGTGGGGAAACCAGCAGGAAGATGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A1Z3N2D4