Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 42843..43368 Replicon plasmid pCAV1043-51
Accession NZ_CP011587
Organism Enterobacter asburiae strain CAV1043

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag AB190_RS00305 Protein ID WP_013023785.1
Coordinates 43063..43368 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag AB190_RS00300 Protein ID WP_001568025.1
Coordinates 42843..43061 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AB190_RS00270 38779..39348 - 570 WP_023332907.1 small heat shock protein sHSP20 -
AB190_RS00275 39383..39664 - 282 WP_023332908.1 helix-turn-helix domain-containing protein -
AB190_RS00280 39858..40121 - 264 WP_001295708.1 hypothetical protein -
AB190_RS00285 40281..41249 + 969 WP_016151349.1 IS5 family transposase -
AB190_RS00290 41600..42016 - 417 WP_032425563.1 type II toxin-antitoxin system VapC family toxin -
AB190_RS00295 42013..42243 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
AB190_RS00300 42843..43061 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
AB190_RS00305 43063..43368 + 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
AB190_RS00310 43555..44559 + 1005 WP_001568027.1 hypothetical protein -
AB190_RS00315 44622..45263 - 642 WP_047741481.1 type-F conjugative transfer system secretin TraK -
AB190_RS26085 45250..45513 - 264 Protein_56 type IV conjugative transfer system protein TraE -
AB190_RS26090 45524..45625 - 102 Protein_57 IS66 family insertion sequence element accessory protein TnpB -
AB190_RS00320 45622..46605 + 984 Protein_58 translesion error-prone DNA polymerase V subunit UmuC -
AB190_RS00325 46687..47661 - 975 WP_021312406.1 ParB/RepB/Spo0J family partition protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..51321 51321
- flank IS/Tn - - 40326..41249 923


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T54634 WP_013023785.1 NZ_CP011587:43063-43368 [Enterobacter asburiae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T54634 NZ_CP011587:43063-43368 [Enterobacter asburiae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACTTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT54634 WP_001568025.1 NZ_CP011587:42843-43061 [Enterobacter asburiae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT54634 NZ_CP011587:42843-43061 [Enterobacter asburiae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGATTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References