Detailed information of TA system TA054633

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 41600..42243
• Replicon: plasmid pCAV1043-51
• Accession: NZ_CP011587
• Organism: Enterobacter asburiae strain CAV1043

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: A0A7D6UII7
• Locus tag: AB190_RS00290
• Protein ID: WP_032425563.1
• Coordinates: 41600..42016 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: AB190_RS00295
• Protein ID: WP_001261276.1
• Coordinates: 42013..42243 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AB190_RS00270	38779..39348	-	570	WP_023332907.1	small heat shock protein sHSP20	-
AB190_RS00275	39383..39664	-	282	WP_023332908.1	helix-turn-helix domain-containing protein	-
AB190_RS00280	39858..40121	-	264	WP_001295708.1	hypothetical protein	-
AB190_RS00285	40281..41249	+	969	WP_016151349.1	IS5 family transposase	-
AB190_RS00290	41600..42016	-	417	WP_032425563.1	type II toxin-antitoxin system VapC family toxin	Toxin
AB190_RS00295	42013..42243	-	231	WP_001261276.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AB190_RS00300	42843..43061	+	219	WP_001568025.1	type II toxin-antitoxin system antitoxin CcdA	-
AB190_RS00305	43063..43368	+	306	WP_013023785.1	type II toxin-antitoxin system toxin CcdB	-
AB190_RS00310	43555..44559	+	1005	WP_001568027.1	hypothetical protein	-
AB190_RS00315	44622..45263	-	642	WP_047741481.1	type-F conjugative transfer system secretin TraK	-
AB190_RS26085	45250..45513	-	264	Protein_56	type IV conjugative transfer system protein TraE	-
AB190_RS26090	45524..45625	-	102	Protein_57	IS66 family insertion sequence element accessory protein TnpB	-
AB190_RS00320	45622..46605	+	984	Protein_58	translesion error-prone DNA polymerase V subunit UmuC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..51321	51321
	-	flank	IS/Tn	-	-	40326..41249	923

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15080.56 Da
• Isoelectric Point: 8.5403

>T54633 WP_032425563.1 NZ_CP011587:c42016-41600 [Enterobacter asburiae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T54633 NZ_CP011587:c42016-41600 [Enterobacter asburiae]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCT
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACCGCCATTGC
CGGACACGCCATCGCGGCGGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCGGGTCTGGTTCTGG
AAGACTGGGTTAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT54633 WP_001261276.1 NZ_CP011587:c42243-42013 [Enterobacter asburiae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT54633 NZ_CP011587:c42243-42013 [Enterobacter asburiae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: A0A7D6UII7

Antitoxin

• AlphaFold DB: A0A387K023