Detailed information of TA system TA054580

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 17302..18038
• Replicon: plasmid pCAV1392-131
• Accession: NZ_CP011577
• Organism: Klebsiella pneumoniae strain CAV1392

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: ABY63_RS00675
• Protein ID: WP_003026803.1
• Coordinates: 17302..17784 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: ABY63_RS00680
• Protein ID: WP_003026799.1
• Coordinates: 17772..18038 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ABY63_RS00655	12939..13901	+	963	WP_004152113.1	M48 family metalloprotease	-
ABY63_RS29910	13989..15243	+	1255	Protein_17	IS3 family transposase	-
ABY63_RS00670	15744..17090	-	1347	WP_020314316.1	ISNCY family transposase	-
ABY63_RS00675	17302..17784	-	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
ABY63_RS00680	17772..18038	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
ABY63_RS00685	18214..18468	-	255	WP_004152108.1	hypothetical protein	-
ABY63_RS00690	18544..18801	-	258	WP_004152107.1	hypothetical protein	-
ABY63_RS00695	18850..19053	-	204	WP_004152106.1	hemolysin expression modulator Hha	-
ABY63_RS00700	19087..19455	-	369	WP_004152105.1	hypothetical protein	-
ABY63_RS00705	19499..19993	-	495	WP_004152104.1	DNA-binding protein	-
ABY63_RS00710	20024..20542	-	519	WP_016197602.1	hypothetical protein	-
ABY63_RS00715	20634..21338	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
ABY63_RS00720	21393..22538	+	1146	Protein_28	TerB family tellurite resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..130719	130719
	-	inside	IScluster/Tn	-	-	14015..21338	7323

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T54580 WP_003026803.1 NZ_CP011577:c17784-17302 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T54580 NZ_CP011577:c17784-17302 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT54580 WP_003026799.1 NZ_CP011577:c18038-17772 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT54580 NZ_CP011577:c18038-17772 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66