Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 22486..23129 Replicon plasmid pCAV1392-50
Accession NZ_CP011576
Organism Klebsiella pneumoniae strain CAV1392

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag ABY63_RS00380 Protein ID WP_001044770.1
Coordinates 22713..23129 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag ABY63_RS00375 Protein ID WP_001261282.1
Coordinates 22486..22716 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ABY63_RS00340 17793..18293 + 501 WP_001239317.1 hypothetical protein -
ABY63_RS00345 18443..19084 + 642 WP_001011939.1 type A-2 chloramphenicol O-acetyltransferase CatII -
ABY63_RS00350 19228..19932 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
ABY63_RS00355 20007..20783 - 777 WP_000015958.1 site-specific integrase -
ABY63_RS00360 20780..21523 - 744 WP_000129823.1 hypothetical protein -
ABY63_RS00365 21574..21924 - 351 WP_000493378.1 hypothetical protein -
ABY63_RS00370 22068..22529 - 462 WP_074193041.1 hypothetical protein -
ABY63_RS00375 22486..22716 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
ABY63_RS00380 22713..23129 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
ABY63_RS00385 23203..23439 + 237 WP_001348075.1 AAA family ATPase -
ABY63_RS00390 23486..24190 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
ABY63_RS00395 24244..25341 + 1098 Protein_28 IS66 family transposase -
ABY63_RS00400 25592..26155 - 564 WP_001097412.1 chlorite dismutase family protein -
ABY63_RS28270 26179..26553 - 375 WP_001348195.1 cupin domain-containing protein -
ABY63_RS00405 26618..27181 - 564 WP_001515734.1 transposase -
ABY63_RS00410 27424..27759 + 336 WP_000046891.1 thermonuclease family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid catA2 / aac(3)-IIa / blaOXA-1 / aac(6')-Ib-cr - 1..49832 49832
- inside IScluster/Tn catA2 / aac(3)-IIa / blaOXA-1 / aac(6')-Ib-cr - 17009..36116 19107


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T54579 WP_001044770.1 NZ_CP011576:22713-23129 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T54579 NZ_CP011576:22713-23129 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT54579 WP_001261282.1 NZ_CP011576:22486-22716 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT54579 NZ_CP011576:22486-22716 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References