Detailed information of TA system TA054545

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3717727..3718484
• Replicon: chromosome
• Accession: NZ_CP011568
• Organism: Pandoraea thiooxydans strain DSM 25325

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: ABW99_RS17135
• Protein ID: WP_206093709.1
• Coordinates: 3717727..3718209 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A0G3ESP8
• Locus tag: ABW99_RS17140
• Protein ID: WP_047214941.1
• Coordinates: 3718212..3718484 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ABW99_RS17125	3714851..3715213	+	363	WP_047214938.1	hypothetical protein	-
ABW99_RS17130	3715355..3717130	-	1776	WP_047214939.1	SWIM zinc finger family protein	-
ABW99_RS17135	3717727..3718209	-	483	WP_206093709.1	GNAT family N-acetyltransferase	Toxin
ABW99_RS17140	3718212..3718484	-	273	WP_047214941.1	DUF1778 domain-containing protein	Antitoxin
ABW99_RS17145	3718898..3719302	+	405	WP_047214942.1	DUF2946 domain-containing protein	-
ABW99_RS17150	3719459..3719839	+	381	WP_047214943.1	copper resistance protein CopC	-
ABW99_RS17155	3719841..3720728	+	888	WP_052892709.1	CopD family protein	-
ABW99_RS17160	3720746..3721711	-	966	WP_047214944.1	NAD-dependent epimerase/dehydratase family protein	-
ABW99_RS17165	3721724..3722926	-	1203	WP_047214945.1	glycine C-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (78-146)

Antitoxin

• Vibrio phage ICP1, Orf50 (3-86)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17460.09 Da
• Isoelectric Point: 9.0966

>T54545 WP_206093709.1 NZ_CP011568:c3718209-3717727 [Pandoraea thiooxydans]
MQLSAPQSLAATHRLDGFNCGEPSLDDWLKRRALANHLNGASRTFVVTDPEQCVLGYYALAAGAVAHQETTSAVRRNMPD
PVPVMVLARLAVDARAQGIKLGAALLQDAVARVQTVAENAGVRALLVHALNDRAKQFYEHYGFRESPVHPMTLMFPMKAR

Nucleotide

• Length: 483 bp

>T54545 NZ_CP011568:c3718209-3717727 [Pandoraea thiooxydans]
ATGCAGCTTTCGGCCCCGCAGTCGCTCGCCGCGACGCATCGACTCGATGGGTTCAATTGCGGAGAGCCATCGCTGGACGA
TTGGTTAAAGCGTCGAGCATTGGCCAACCACCTGAACGGGGCAAGCCGCACCTTCGTCGTGACTGATCCTGAACAGTGCG
TACTTGGGTACTATGCTTTAGCAGCGGGGGCCGTCGCGCACCAGGAAACCACGAGCGCCGTCAGGCGCAATATGCCCGAT
CCGGTGCCGGTGATGGTGCTCGCCAGGCTCGCGGTAGATGCTCGCGCCCAAGGCATCAAACTCGGTGCGGCTTTGCTCCA
AGATGCTGTAGCTCGGGTGCAGACCGTTGCCGAGAATGCTGGTGTTCGGGCGCTGCTGGTCCATGCGTTGAACGATCGCG
CAAAGCAGTTCTATGAGCACTATGGTTTCCGGGAGTCGCCAGTTCACCCGATGACGTTGATGTTCCCCATGAAAGCACGG
TAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10072.53 Da
• Isoelectric Point: 5.8189

>AT54545 WP_047214941.1 NZ_CP011568:c3718484-3718212 [Pandoraea thiooxydans]
MRDASINLRALPEQRDLIDHAASLLGKNRSDFMLEAACERAKAIVLDQVFFNLDESRFRQFTALLDAPQDANPGLERLMA
IKAPWSASKA

Nucleotide

• Length: 273 bp

>AT54545 NZ_CP011568:c3718484-3718212 [Pandoraea thiooxydans]
ATGCGTGACGCCTCCATCAACTTACGTGCGCTGCCCGAGCAGCGCGACTTGATCGACCATGCCGCCAGTCTGCTGGGCAA
AAATCGCTCAGACTTCATGCTGGAAGCGGCGTGTGAACGCGCGAAGGCGATTGTGCTGGATCAGGTTTTTTTTAACCTGG
ACGAGAGCCGATTCCGGCAGTTCACTGCACTACTCGATGCGCCACAAGATGCCAATCCGGGCCTTGAACGGCTGATGGCG
ATTAAGGCACCGTGGAGCGCAAGTAAGGCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0G3ESP8