Detailed information of TA system TA054518

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2275950..2276581
• Replicon: chromosome
• Accession: NZ_CP011539
• Organism: Klebsiella aerogenes strain G7

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1Q8YTV3
• Locus tag: EAG7_RS10820
• Protein ID: WP_012542177.1
• Coordinates: 2276405..2276581 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: EAG7_RS10815
• Protein ID: WP_041165571.1
• Coordinates: 2275950..2276357 (-)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EAG7_RS10775	2271890..2272435	-	546	WP_015367382.1	terminase small subunit	-
EAG7_RS10780	2272729..2273148	-	420	WP_015367383.1	hypothetical protein	-
EAG7_RS10785	2273148..2273474	-	327	WP_015367384.1	hypothetical protein	-
EAG7_RS10790	2273646..2273873	+	228	WP_134874998.1	hypothetical protein	-
EAG7_RS10800	2274553..2275029	-	477	WP_173390334.1	DUF2514 domain-containing protein	-
EAG7_RS10805	2275062..2275595	-	534	WP_015367388.1	lysozyme	-
EAG7_RS10810	2275597..2275845	-	249	WP_032706073.1	class II holin family protein	-
EAG7_RS10815	2275950..2276357	-	408	WP_041165571.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
EAG7_RS10820	2276405..2276581	-	177	WP_012542177.1	type II toxin-antitoxin system HicA family toxin	Toxin
EAG7_RS10825	2276949..2277551	-	603	WP_041165614.1	hypothetical protein	-
EAG7_RS10830	2277565..2278596	-	1032	WP_015367392.1	DUF968 domain-containing protein	-
EAG7_RS10835	2278596..2278799	-	204	WP_015367393.1	hypothetical protein	-
EAG7_RS10840	2278796..2279188	-	393	WP_015367394.1	SOS-response repressor and protease LexA	-
EAG7_RS10850	2279531..2279764	-	234	WP_015367395.1	DinI family protein	-
EAG7_RS10855	2279958..2280755	-	798	WP_041165572.1	hypothetical protein	-
EAG7_RS10860	2280765..2280959	-	195	WP_015367397.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2238636..2292550	53914

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-61)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6632.83 Da
• Isoelectric Point: 11.0174

>T54518 WP_012542177.1 NZ_CP011539:c2276581-2276405 [Klebsiella aerogenes]
VKQSELRRWLAAQGAEFKDGTNHLKIYLNGKQTVMPRHPGKEIPEPLRKAILKQLGIK

Nucleotide

• Length: 177 bp

>T54518 NZ_CP011539:c2276581-2276405 [Klebsiella aerogenes]
GTGAAGCAAAGCGAGTTGAGACGTTGGCTTGCAGCTCAAGGGGCAGAGTTTAAAGATGGTACTAACCACCTGAAAATCTA
CCTCAACGGCAAGCAAACGGTAATGCCGAGACATCCGGGGAAGGAAATACCGGAACCGCTGAGGAAGGCAATTCTGAAGC
AACTTGGCATCAAATAA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 15020.03 Da
• Isoelectric Point: 4.4368

>AT54518 WP_041165571.1 NZ_CP011539:c2276357-2275950 [Klebsiella aerogenes]
MRYPVIFEHDETGWAVFFPDIPEAMTGGETREEALEMAQDALVTAFDFYFDDRREIPAPSADGEAFVEVPSSVAAKVLLL
NRLVSTNTSNAELARMINTRPQEVQRIVSLGHSTKIDTIQKALAALGQHMEIVVR

Nucleotide

• Length: 408 bp

>AT54518 NZ_CP011539:c2276357-2275950 [Klebsiella aerogenes]
ATGCGATACCCGGTAATTTTTGAACATGATGAAACCGGCTGGGCGGTATTCTTTCCGGATATCCCAGAGGCAATGACGGG
CGGAGAGACCAGGGAGGAAGCGTTAGAAATGGCGCAAGATGCCCTGGTAACGGCGTTTGATTTCTACTTCGACGATCGCC
GGGAGATTCCCGCGCCATCAGCTGACGGTGAGGCATTTGTTGAGGTTCCGTCCAGCGTAGCGGCGAAGGTATTGCTGCTA
AATCGTCTGGTAAGCACCAACACCAGCAATGCTGAACTTGCCCGCATGATTAATACGCGTCCGCAGGAAGTACAGCGCAT
CGTATCGCTTGGTCACAGCACCAAAATTGATACGATTCAAAAAGCTCTGGCAGCGCTGGGACAGCATATGGAAATTGTCG
TTCGTTAA

Structures

Toxin

• AlphaFold DB: A0A1Q8YTV3