54497

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 510889..511525 Replicon chromosome
Accession NZ_CP011534
Organism Bacillus subtilis strain UD1022

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag ABA10_RS02630 Protein ID WP_003156187.1
Coordinates 511175..511525 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag ABA10_RS02625 Protein ID WP_003225183.1
Coordinates 510889..511170 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ABA10_RS02605 507248..507847 - 600 WP_015482831.1 rhomboid family intramembrane serine protease -
ABA10_RS02610 507942..508307 + 366 WP_015252768.1 holo-ACP synthase -
ABA10_RS02615 508473..509489 + 1017 WP_080030712.1 outer membrane lipoprotein carrier protein LolA -
ABA10_RS02620 509604..510773 + 1170 WP_015252766.1 alanine racemase -
ABA10_RS02625 510889..511170 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
ABA10_RS02630 511175..511525 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
ABA10_RS02635 511641..512465 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
ABA10_RS02640 512470..512835 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
ABA10_RS02645 512839..513240 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
ABA10_RS02650 513252..514259 + 1008 WP_047182124.1 phosphoserine phosphatase RsbU -
ABA10_RS02655 514321..514650 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
ABA10_RS02660 514647..515129 + 483 WP_003234299.1 anti-sigma B factor RsbW -
ABA10_RS02665 515095..515883 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
ABA10_RS02670 515883..516482 + 600 WP_015382836.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T54497 WP_003156187.1 NZ_CP011534:511175-511525 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T54497 NZ_CP011534:511175-511525 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT54497 WP_003225183.1 NZ_CP011534:510889-511170 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT54497 NZ_CP011534:510889-511170 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB G4NU32

References