Detailed information of TA system TA054484

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2063923..2064452
• Replicon: chromosome
• Accession: NZ_CP011528
• Organism: Staphylococcus aureus strain RKI4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: AA961_RS10430
• Protein ID: WP_000621175.1
• Coordinates: 2063923..2064285 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: AA961_RS10435
• Protein ID: WP_000948331.1
• Coordinates: 2064282..2064452 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AA961_RS10405	2060901..2061671	-	771	WP_001041102.1	RNA polymerase sigma factor SigB	-
AA961_RS10410	2061646..2062125	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
AA961_RS10415	2062127..2062453	-	327	WP_001052491.1	anti-sigma factor antagonist	-
AA961_RS10420	2062572..2063573	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
AA961_RS10430	2063923..2064285	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AA961_RS10435	2064282..2064452	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
AA961_RS10440	2064537..2065685	-	1149	WP_047928568.1	alanine racemase	-
AA961_RS10445	2065751..2066110	-	360	WP_000581197.1	holo-ACP synthase	-
AA961_RS10450	2066114..2066605	-	492	WP_072353916.1	PH domain-containing protein	-
AA961_RS10455	2066592..2068175	-	1584	WP_001294626.1	PH domain-containing protein	-
AA961_RS10460	2068168..2068647	-	480	WP_031590204.1	hypothetical protein	-
AA961_RS10465	2068856..2069416	-	561	WP_001092410.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T54484 WP_000621175.1 NZ_CP011528:c2064285-2063923 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T54484 NZ_CP011528:c2064285-2063923 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT54484 WP_000948331.1 NZ_CP011528:c2064452-2064282 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT54484 NZ_CP011528:c2064452-2064282 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1