Detailed information of TA system TA054464

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 5635627..5636261
• Replicon: chromosome
• Accession: NZ_CP011525
• Organism: Pseudomonas sp. JY-Q

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: L1LRL5
• Locus tag: AA098_RS25405
• Protein ID: WP_009408500.1
• Coordinates: 5635627..5636031 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: L1LS63
• Locus tag: AA098_RS25410
• Protein ID: WP_003285520.1
• Coordinates: 5636031..5636261 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AA098_RS25380	5631021..5631854	-	834	WP_003255528.1	indole-3-glycerol phosphate synthase TrpC	-
AA098_RS25385	5631851..5632900	-	1050	WP_003255529.1	anthranilate phosphoribosyltransferase	-
AA098_RS25390	5632910..5633503	-	594	WP_004574924.1	aminodeoxychorismate/anthranilate synthase component II	-
AA098_RS25400	5634572..5635540	+	969	WP_009408501.1	DUF932 domain-containing protein	-
AA098_RS25405	5635627..5636031	-	405	WP_009408500.1	tRNA(fMet)-specific endonuclease VapC	Toxin
AA098_RS25410	5636031..5636261	-	231	WP_003285520.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AA098_RS25415	5636418..5637422	+	1005	WP_064615083.1	YqaJ viral recombinase family protein	-
AA098_RS25420	5637516..5638466	+	951	WP_064615084.1	hypothetical protein	-
AA098_RS25425	5638438..5638935	+	498	WP_009408491.1	DNA repair protein RadC	-
AA098_RS29620	5638945..5639121	+	177	WP_009408489.1	hypothetical protein	-
AA098_RS25430	5639405..5639983	-	579	WP_003291613.1	plasmid pRiA4b ORF-3 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-35)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14977.20 Da
• Isoelectric Point: 7.2774

>T54464 WP_009408500.1 NZ_CP011525:c5636031-5635627 [Pseudomonas sp. JY-Q]
MLKYMLDTNICIFTIKNRPEQVREAFKRHSGQLSISTVTLMELIYGAEKSANPERNLADVEGFAARLEVLPYDAQAAAHS
GQLRAELARIGKPIGPYDQMIAGHARAQGLILVTNNLREFERVPGLRVEDWVNA

Nucleotide

• Length: 405 bp

>T54464 NZ_CP011525:c5636031-5635627 [Pseudomonas sp. JY-Q]
ATGCTGAAGTACATGCTTGATACCAACATCTGCATTTTCACGATCAAGAATCGTCCTGAGCAGGTGCGGGAAGCCTTCAA
GCGCCATTCAGGGCAGCTGAGCATCAGCACTGTCACCCTGATGGAGCTTATTTACGGCGCCGAGAAGTCCGCTAATCCCG
AGCGTAATCTGGCGGACGTTGAAGGTTTTGCTGCGCGCCTGGAAGTACTGCCTTACGACGCTCAGGCAGCGGCTCATTCC
GGGCAATTGCGAGCTGAGTTGGCTCGTATCGGCAAGCCTATTGGCCCGTATGATCAGATGATTGCAGGACACGCACGTGC
CCAGGGACTGATTCTGGTCACGAATAATCTTCGTGAGTTCGAGCGAGTCCCAGGTTTGCGCGTCGAGGATTGGGTGAACG
CCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8432.41 Da
• Isoelectric Point: 4.2683

>AT54464 WP_003285520.1 NZ_CP011525:c5636261-5636031 [Pseudomonas sp. JY-Q]
MEQGAVFQSNRSQAVRLPKAVALPEDVKRVDVVVVGRARIITPAGEAWDVWFDGEGVTADYMADREQPADQERDAF

Nucleotide

• Length: 231 bp

>AT54464 NZ_CP011525:c5636261-5636031 [Pseudomonas sp. JY-Q]
ATGGAGCAAGGTGCAGTTTTTCAGAGCAACCGCAGTCAGGCGGTGCGTTTGCCCAAGGCGGTTGCGCTGCCTGAGGATGT
GAAGCGGGTGGATGTAGTGGTAGTTGGTCGAGCCCGAATCATCACGCCGGCAGGTGAGGCCTGGGACGTCTGGTTCGATG
GCGAAGGTGTGACCGCTGACTACATGGCGGATCGCGAGCAGCCTGCAGATCAAGAGCGGGATGCGTTCTGA

Structures

Toxin

• AlphaFold DB: L1LRL5

Antitoxin

• AlphaFold DB: L1LS63