Detailed information of TA system TA054445

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-mazE/MazF(toxin)
• Location: 1522027..1522635
• Replicon: chromosome
• Accession: NZ_CP011512
• Organism: Paenibacillus peoriae strain HS311

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: H6CFY1
• Locus tag: ABE82_RS06440
• Protein ID: WP_007429335.1
• Coordinates: 1522285..1522635 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: ABE82_RS06435
• Protein ID: WP_170970806.1
• Coordinates: 1522027..1522278 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ABE82_RS06420	1518228..1518860	+	633	WP_053324896.1	LysE family transporter	-
ABE82_RS06425	1518971..1520248	+	1278	WP_023987594.1	outer membrane lipoprotein carrier protein LolA	-
ABE82_RS06430	1520507..1521703	+	1197	WP_028543050.1	alanine racemase	-
ABE82_RS06435	1522027..1522278	+	252	WP_170970806.1	ribbon-helix-helix protein, CopG family	Antitoxin
ABE82_RS06440	1522285..1522635	+	351	WP_007429335.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ABE82_RS06445	1522815..1525037	+	2223	WP_179085377.1	RNA-binding transcriptional accessory protein	-
ABE82_RS27810	1525141..1525275	-	135	WP_007429337.1	cortex morphogenetic protein CmpA	-
ABE82_RS06450	1525420..1525944	+	525	WP_053324897.1	SprT family protein	-
ABE82_RS06465	1526333..1527208	-	876	WP_023987597.1	Cof-type HAD-IIB family hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-36)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12822.77 Da
• Isoelectric Point: 5.6195

>T54445 WP_007429335.1 NZ_CP011512:1522285-1522635 [Paenibacillus peoriae]
MIVKRGDVFFADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAATHGFDRDSVVLLEQI
RTIDKQRLTDKITHLDEETMRKVSDSLQISLGLIDF

Nucleotide

• Length: 351 bp

>T54445 NZ_CP011512:1522285-1522635 [Paenibacillus peoriae]
TTGATCGTAAAGCGCGGCGACGTTTTTTTTGCAGACCTTTCACCCGTAGTCGGTTCCGAGCAAGGTGGCGTCAGACCTGT
GCTGATCATTCAAAATGATATCGGCAACCGGTTTAGTCCTACTGTGATCGTAGCAGCTATCACCGCTCAGATTCAGAAGG
CAAAGCTGCCTACACACGTGGAAATCGACGCGGCGACGCATGGCTTTGATCGTGATTCTGTTGTTCTTCTGGAGCAAATT
CGGACGATCGACAAGCAAAGGCTTACCGACAAAATCACCCATTTAGATGAAGAAACCATGCGCAAAGTGAGCGATTCGCT
ACAAATCAGTCTCGGTTTGATTGATTTTTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9465.86 Da
• Isoelectric Point: 6.4671

>AT54445 WP_170970806.1 NZ_CP011512:1522027-1522278 [Paenibacillus peoriae]
MISLPDHLLQEVDGIVAMENSNRSEFIRQAMKLYVGERKKRYIREAMQRGYMEMAKINLTMASEAFHAEEDANSTLGRTV
SGV

Nucleotide

• Length: 252 bp

>AT54445 NZ_CP011512:1522027-1522278 [Paenibacillus peoriae]
ATGATCAGTTTGCCAGATCATCTCTTGCAGGAAGTGGACGGGATCGTAGCTATGGAAAATTCCAACCGCAGTGAATTTAT
CAGGCAGGCCATGAAGCTGTATGTAGGCGAACGTAAAAAACGTTACATTCGTGAAGCTATGCAGCGTGGATACATGGAAA
TGGCTAAAATCAACTTAACCATGGCATCCGAAGCCTTTCACGCGGAGGAAGATGCAAACAGCACCTTGGGCCGTACAGTT
AGCGGGGTGTAA

Structures

Toxin

• AlphaFold DB: H6CFY1