Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 4048965..4049639 Replicon chromosome
Accession NZ_CP011510
Organism Mycobacterium tuberculosis strain Beijing

Toxin (Protein)


Gene name vapC Uniprot ID P9WF52
Locus tag TM57_RS18865 Protein ID WP_003417282.1
Coordinates 4049211..4049639 (+) Length 143 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TI59
Locus tag TM57_RS18860 Protein ID WP_003417286.1
Coordinates 4048965..4049207 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
TM57_RS18835 4045501..4046565 + 1065 WP_003913037.1 GTP 3',8-cyclase MoaA -
TM57_RS18840 4046662..4047036 + 375 WP_003417295.1 4a-hydroxytetrahydrobiopterin dehydratase -
TM57_RS18845 4047033..4047566 + 534 WP_003417293.1 cyclic pyranopterin monophosphate synthase MoaC -
TM57_RS18850 4047567..4048232 + 666 WP_003417290.1 molybdopterin converting factor subunit 1 -
TM57_RS18855 4048229..4048843 + 615 WP_003417288.1 class I SAM-dependent methyltransferase -
TM57_RS18860 4048965..4049207 + 243 WP_003417286.1 ribbon-helix-helix domain-containing protein Antitoxin
TM57_RS18865 4049211..4049639 + 429 WP_003417282.1 type II toxin-antitoxin system VapC family toxin Toxin
TM57_RS18870 4049718..4050509 - 792 WP_003417279.1 succinate dehydrogenase iron-sulfur subunit -
TM57_RS18875 4050509..4052281 - 1773 WP_003417273.1 succinate dehydrogenase flavoprotein subunit -
TM57_RS18880 4052410..4052844 - 435 WP_003902441.1 succinate dehydrogenase hydrophobic membrane anchor subunit -
TM57_RS18885 4052841..4053275 - 435 WP_003900017.1 succinate dehydrogenase, cytochrome b556 subunit -
TM57_RS18890 4053416..4053817 + 402 WP_003417264.1 cytidine deaminase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-141)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 143 a.a.        Molecular weight: 15834.16 Da        Isoelectric Point: 8.5471

>T54429 WP_003417282.1 NZ_CP011510:4049211-4049639 [Mycobacterium tuberculosis]
MRALLDVNVLLALLDRDHVDHERARAWITGQIERGWASCAITQNGFVRVISQPRYPSPISVAHAIDLLARATHTRYHEFW
SCTVSILDSKVIDRSRLHSPKQVTDAYLLALAVAHDGRFVTFDQSIALTAVPGATKQHLATL

Download         Length: 429 bp

>T54429 NZ_CP011510:4049211-4049639 [Mycobacterium tuberculosis]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8849.86 Da        Isoelectric Point: 5.1007

>AT54429 WP_003417286.1 NZ_CP011510:4048965-4049207 [Mycobacterium tuberculosis]
MRTTLSIDDDVLLAVKERARREKRTAGEILSDLARQALTNQNPQPAASQEDAFHGFEPLPHRGGAVSNALIDRLRDEEAV

Download         Length: 243 bp

>AT54429 NZ_CP011510:4048965-4049207 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBL0

Antitoxin

Source ID Structure
AlphaFold DB G0TI59

References