Detailed information of TA system TA054423

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Rv0299-vapB/-
• Location: 2975203..2975774
• Replicon: chromosome
• Accession: NZ_CP011510
• Organism: Mycobacterium tuberculosis strain Beijing

Toxin (Protein)

• Gene name: Rv0299
• Uniprot ID: -
• Locus tag: TM57_RS14145
• Protein ID: WP_003401560.1
• Coordinates: 2975472..2975774 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: O07227
• Locus tag: TM57_RS14140
• Protein ID: WP_003401563.1
• Coordinates: 2975203..2975424 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TM57_RS14125	2973108..2974016	-	909	WP_003900117.1	SDR family NAD(P)-dependent oxidoreductase	-
TM57_RS14130	2974013..2974645	-	633	WP_003401571.1	TetR/AcrR family transcriptional regulator	-
TM57_RS14135	2974781..2975206	-	426	WP_003401566.1	PIN domain nuclease	-
TM57_RS14140	2975203..2975424	-	222	WP_003401563.1	type II toxin-antitoxin system antitoxin VapB2	Antitoxin
TM57_RS14145	2975472..2975774	-	303	WP_003401560.1	toxin-antitoxin system toxin	Toxin
TM57_RS14150	2975771..2975998	-	228	WP_003401555.1	ribbon-helix-helix protein, CopG family	-
TM57_RS14155	2976141..2977916	-	1776	WP_010886074.1	PE family protein	-
TM57_RS14160	2978095..2979492	+	1398	WP_003401544.1	sulfatase	-
TM57_RS14165	2979502..2980305	+	804	WP_003401540.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Ribbon-helix-helix protein, CopG (9-37)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10442.16 Da
• Isoelectric Point: 4.9609

>T54423 WP_003401560.1 NZ_CP011510:c2975774-2975472 [Mycobacterium tuberculosis]
MIAPGDIAPRRDSEHELYVAVLSNALHRAADTGRVITCPFIPGRVPEDLLAMVVAVEQPNGTLLPELVQWLHVAALGAPL
GNAGVAALREAASVVTALLC

Nucleotide

• Length: 303 bp

>T54423 NZ_CP011510:c2975774-2975472 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8088.31 Da
• Isoelectric Point: 10.7177

>AT54423 WP_003401563.1 NZ_CP011510:c2975424-2975203 [Mycobacterium tuberculosis]
MSDVLIRDIPDDVLASLDAIAARLGLSRTEYIRRRLAQDAQTARVTVTAADLRRLRGAVAGLGDPELMRQAWR

Nucleotide

• Length: 222 bp

>AT54423 NZ_CP011510:c2975424-2975203 [Mycobacterium tuberculosis]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Structures

Antitoxin

• PDB: 3H87
• AlphaFold DB: A0A829CG48