Detailed information of TA system TA054421

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-ParD
• Location: 2894344..2895355
• Replicon: chromosome
• Accession: NZ_CP011510
• Organism: Mycobacterium tuberculosis strain Beijing

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: L7N5R2
• Locus tag: TM57_RS13815
• Protein ID: WP_003401859.1
• Coordinates: 2894762..2895355 (+)
• Length: 198 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: O53702
• Locus tag: TM57_RS13810
• Protein ID: WP_003401860.1
• Coordinates: 2894344..2894733 (+)
• Length: 130 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TM57_RS13785	2890192..2890947	+	756	WP_003401865.1	XdhC family protein	-
TM57_RS13790	2890944..2891537	+	594	WP_003401864.1	NTP transferase domain-containing protein	-
TM57_RS13795	2891534..2892430	+	897	WP_003898418.1	MoxR family ATPase	-
TM57_RS13800	2892444..2893046	+	603	WP_003898417.1	SRPBCC family protein	-
TM57_RS13805	2893052..2894263	+	1212	WP_003401861.1	VWA domain-containing protein	-
TM57_RS13810	2894344..2894733	+	390	WP_003401860.1	hypothetical protein	Antitoxin
TM57_RS13815	2894762..2895355	+	594	WP_003401859.1	zeta toxin family protein	Toxin
TM57_RS13820	2895380..2896510	+	1131	WP_003401858.1	hypothetical protein	-
TM57_RS13825	2896499..2897182	-	684	WP_003401857.1	VTT domain-containing protein	-
TM57_RS13830	2897278..2898312	+	1035	WP_003401856.1	class II fructose-bisphosphate aldolase	-
TM57_RS13835	2898324..2899706	-	1383	WP_003898415.1	magnesium transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (4-156)

Antitoxin

• ParD-like antitoxin of type II bacterial toxin-antitoxin system (6-106)

Sequences

Toxin

Protein

• Length: 198 a.a.
• Molecular weight: 21344.53 Da
• Isoelectric Point: 10.5018

>T54421 WP_003401859.1 NZ_CP011510:2894762-2895355 [Mycobacterium tuberculosis]
VKRLDLVAGPNGAGKSTFVALTLAPLLPGIVFVNADEIAKQRWPDDPTSHAYQAAQVAADTRARLIDLGRPFIAETVFSH
PSKLELIRTARTAGYTVVLHVLVIPEGLAVERVRHRVAAGGHDVPETKIRERHRRLAELVAQAITLADGATVYDNSRLAG
PRIVAQFSGGGIIGRACWPSWTPPPLMSRWSNRPETA

Nucleotide

• Length: 594 bp

>T54421 NZ_CP011510:2894762-2895355 [Mycobacterium tuberculosis]
GTGAAGCGGCTCGATCTGGTCGCCGGGCCCAACGGCGCCGGCAAGTCGACGTTCGTCGCCCTCACGCTGGCGCCCTTGCT
GCCCGGCATCGTCTTCGTCAACGCCGACGAAATCGCCAAACAACGCTGGCCCGACGACCCAACATCGCACGCCTACCAGG
CGGCGCAGGTCGCCGCCGACACCCGCGCGAGGCTCATCGACTTGGGCCGGCCGTTCATTGCCGAGACGGTGTTCTCGCAC
CCATCGAAGCTCGAGCTCATCCGCACCGCGCGCACGGCCGGCTACACCGTCGTACTGCACGTGTTGGTTATCCCCGAAGG
CCTGGCGGTCGAGCGCGTCAGGCATCGCGTCGCCGCGGGCGGCCACGATGTGCCGGAGACCAAGATCCGCGAGCGTCACC
GCCGGCTGGCCGAGCTTGTCGCCCAGGCCATCACGCTGGCCGACGGCGCCACCGTCTATGACAACAGCCGGCTCGCGGGC
CCGCGGATCGTCGCTCAGTTCAGCGGGGGTGGCATCATCGGGCGCGCATGCTGGCCTTCGTGGACGCCGCCACCCTTGAT
GTCACGCTGGAGTAACAGGCCTGAGACGGCGTAA

Antitoxin

Protein

• Length: 130 a.a.
• Molecular weight: 13648.21 Da
• Isoelectric Point: 6.0829

>AT54421 WP_003401860.1 NZ_CP011510:2894344-2894733 [Mycobacterium tuberculosis]
VPKAVDRVTRVAADLVDSAAAEGARQSRSAKQQLDHWARVGRAVSNQHTASRRRVEAALAGHLPMTDLTLEEGVVFNAEI
SAAIEERLSRTNYGDVLAAQGITTVALNDAGDIVEHRPDGTSVVLAATP

Nucleotide

• Length: 390 bp

>AT54421 NZ_CP011510:2894344-2894733 [Mycobacterium tuberculosis]
GTGCCCAAAGCCGTCGACCGCGTCACGCGCGTCGCCGCGGATCTGGTGGACAGCGCGGCGGCCGAGGGTGCTCGTCAGAG
CCGTTCGGCCAAGCAGCAGCTCGACCACTGGGCCCGCGTCGGTCGGGCGGTGTCGAACCAGCACACGGCGTCGCGGCGCC
GCGTGGAAGCTGCGCTCGCGGGGCATCTGCCGATGACGGACCTGACGCTGGAGGAGGGCGTGGTGTTCAACGCCGAGATC
TCCGCAGCTATCGAGGAGCGCCTGTCGCGAACCAACTACGGCGACGTGCTCGCCGCGCAGGGCATCACCACGGTCGCCCT
GAACGACGCGGGCGACATCGTCGAGCACCGGCCAGACGGCACCTCGGTCGTGCTGGCGGCCACGCCGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4BRU8

Antitoxin

• AlphaFold DB: A0A7U4BRT0