Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-PHD
Location 2645392..2646038 Replicon chromosome
Accession NZ_CP011510
Organism Mycobacterium tuberculosis strain Beijing

Toxin (Protein)


Gene name vapC Uniprot ID O07783
Locus tag TM57_RS12635 Protein ID WP_003403122.1
Coordinates 2645646..2646038 (+) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TQ86
Locus tag TM57_RS12630 Protein ID WP_003403125.1
Coordinates 2645392..2645649 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
TM57_RS12595 2640710..2641021 - 312 WP_003403164.1 hypothetical protein -
TM57_RS12600 2641078..2641839 + 762 WP_003403148.1 two-component system response regulator TcrA -
TM57_RS12605 2641823..2642353 + 531 WP_003917320.1 two component system sensor kinase HK2 -
TM57_RS12610 2642467..2642973 + 507 WP_003900973.1 two component system sensor kinase HK1 -
TM57_RS12615 2643077..2643313 + 237 WP_003403139.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
TM57_RS12620 2643310..2643723 + 414 WP_003403137.1 type II toxin-antitoxin system VapC family toxin -
TM57_RS12625 2643974..2645209 + 1236 WP_003403128.1 ATP-binding protein -
TM57_RS12630 2645392..2645649 + 258 WP_003403125.1 type II toxin-antitoxin system antitoxin VapB4 Antitoxin
TM57_RS12635 2645646..2646038 + 393 WP_003403122.1 type II toxin-antitoxin system toxin ribonuclease C4 Toxin
TM57_RS12640 2646090..2647640 - 1551 WP_003905349.1 MCE family protein -
TM57_RS12645 2647645..2648787 - 1143 WP_003911253.1 virulence factor Mce family protein -
TM57_RS12650 2648850..2650376 - 1527 WP_003403112.1 virulence factor Mce family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(8-122)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(3-53)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14080.08 Da        Isoelectric Point: 4.6558

>T54418 WP_003403122.1 NZ_CP011510:2645646-2646038 [Mycobacterium tuberculosis]
VNVRRALADTSVFIGIEATRFDPDRFAGYEWGVSVVTLGELRLGVLQASGPEAAARRLSTYQLAQRFEPLGIDEAVSEAW
ALLVSKLRAAKLRVPINDSWIAATAVAHGIAILTQDNDYAAMPDVEVITI

Download         Length: 393 bp

>T54418 NZ_CP011510:2645646-2646038 [Mycobacterium tuberculosis]
GTGAACGTACGGCGCGCCCTCGCCGACACATCCGTTTTCATTGGCATCGAGGCGACACGATTCGACCCGGACCGGTTTGC
GGGATACGAATGGGGCGTCTCGGTAGTCACACTCGGCGAGCTGCGGCTCGGGGTGTTGCAAGCCTCCGGCCCCGAGGCCG
CCGCACGTCGGCTCTCCACCTACCAGCTTGCGCAACGGTTCGAACCACTAGGCATCGACGAGGCGGTCTCCGAAGCATGG
GCATTACTGGTGTCCAAGCTGCGCGCCGCCAAACTGCGCGTGCCGATCAACGACAGCTGGATTGCGGCCACAGCCGTGGC
GCACGGCATTGCGATCCTGACCCAAGACAACGACTACGCCGCCATGCCCGACGTCGAGGTCATAACGATCTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9681.06 Da        Isoelectric Point: 7.0175

>AT54418 WP_003403125.1 NZ_CP011510:2645392-2645649 [Mycobacterium tuberculosis]
MSATIPARDLRNHTAEVLRRVAAGEEIEVLKDNRPVARIVPLKRRRQWLPAAEVIGELVRLGPDTTNLGEELRETLTQTT
DDVRW

Download         Length: 258 bp

>AT54418 NZ_CP011510:2645392-2645649 [Mycobacterium tuberculosis]
ATGTCTGCTACGATACCCGCTCGCGATCTTCGCAACCACACCGCCGAGGTACTGCGGCGAGTTGCCGCCGGCGAGGAAAT
CGAGGTGCTCAAGGACAATCGCCCCGTAGCGCGCATCGTTCCGCTCAAGCGGCGCCGCCAATGGTTGCCAGCTGCCGAGG
TGATCGGCGAACTGGTGCGCTTGGGCCCCGATACCACCAATCTGGGCGAGGAGCTGCGAGAGACGCTGACGCAAACCACG
GACGATGTGCGGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F8V4

Antitoxin

Source ID Structure
AlphaFold DB G0TQ86

References