Detailed information of TA system TA054417

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 2643077..2643723
• Replicon: chromosome
• Accession: NZ_CP011510
• Organism: Mycobacterium tuberculosis strain Beijing

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: TM57_RS12620
• Protein ID: WP_003403137.1
• Coordinates: 2643310..2643723 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: TM57_RS12615
• Protein ID: WP_003403139.1
• Coordinates: 2643077..2643313 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TM57_RS12580	2638151..2638861	-	711	Protein_2456	transposase	-
TM57_RS12585	2638863..2639447	-	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
TM57_RS12590	2639688..2640638	-	951	WP_003900184.1	DUF1259 domain-containing protein	-
TM57_RS12595	2640710..2641021	-	312	WP_003403164.1	hypothetical protein	-
TM57_RS12600	2641078..2641839	+	762	WP_003403148.1	two-component system response regulator TcrA	-
TM57_RS12605	2641823..2642353	+	531	WP_003917320.1	two component system sensor kinase HK2	-
TM57_RS12610	2642467..2642973	+	507	WP_003900973.1	two component system sensor kinase HK1	-
TM57_RS12615	2643077..2643313	+	237	WP_003403139.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
TM57_RS12620	2643310..2643723	+	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
TM57_RS12625	2643974..2645209	+	1236	WP_003403128.1	ATP-binding protein	-
TM57_RS12630	2645392..2645649	+	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
TM57_RS12635	2645646..2646038	+	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
TM57_RS12640	2646090..2647640	-	1551	WP_003905349.1	MCE family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T54417 WP_003403137.1 NZ_CP011510:2643310-2643723 [Mycobacterium tuberculosis]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T54417 NZ_CP011510:2643310-2643723 [Mycobacterium tuberculosis]
GTGAAACCGCCGCTGGCAGTGGACACCAGCGTCGCGATTCCGTTGCTGGTACGGACCCATACCGCGCATGCGGCGGTTGT
CGCCTGGTGGGCACATCGGGAGGCCGCGCTGTGCGGGCACGCCCTGGCCGAAACCTACTCCGTGCTGACCCGCCTGCCGC
GTGACCTACGACTGGCACCGATGGACGCCGCGCGGCTTCTTACCGAACGCTTTGCGGCACCGCTGTTGTTGAGTTCCCGC
ACCACTGAACATCTCCCACGCGTGCTGGCCCAATTCGAGATCACTGGTGGCGCGGTCTATGACGCTCTCGTGGCCCTTGC
CGCCGCCGAGCACCGGGCTGAACTCGCCACACGCGATGCTCGAGCCAAGGACACTTACGAAAAGATCGGCGTCCACGTCG
TTGTCGCCGCGTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT54417 WP_003403139.1 NZ_CP011510:2643077-2643313 [Mycobacterium tuberculosis]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT54417 NZ_CP011510:2643077-2643313 [Mycobacterium tuberculosis]
ATGAAGGCTGTTGTGGATGCGGCTGGCCGCATCGTCGTACCCAAACCGCTGCGCGAAGCCTTGGGGCTGCAACCGGGGTC
CACCGTGGAAATATCCCGCTATGGCGCTGGTCTCCACCTGATTCCCACCGGACGAACAGCGCGTCTCGAAGAGGAGAATG
GCGTACTGGTGGCCACCGGTGAGACCACGATCGACGATGAGGTCGTGTTCGGCCTTATCGATAGCGGCCGGAAGTGA

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89