Detailed information of TA system TA054404

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2104753..2105321
• Replicon: chromosome
• Accession: NZ_CP011510
• Organism: Mycobacterium tuberculosis strain Beijing

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: TM57_RS09825
• Protein ID: WP_003405865.1
• Coordinates: 2104753..2105127 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: TM57_RS09830
• Protein ID: WP_003405863.1
• Coordinates: 2105124..2105321 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TM57_RS09790	2101166..2101873	+	708	Protein_1923	adenylate/guanylate cyclase domain-containing protein	-
TM57_RS22190	2101825..2102034	-	210	WP_003911400.1	hypothetical protein	-
TM57_RS09800	2102056..2102766	+	711	WP_003911399.1	hypothetical protein	-
TM57_RS09805	2102781..2103104	-	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
TM57_RS09810	2103347..2103622	+	276	WP_003405867.1	hypothetical protein	-
TM57_RS09815	2103549..2103734	-	186	WP_003901093.1	hypothetical protein	-
TM57_RS09820	2103852..2104550	-	699	WP_003898733.1	hypothetical protein	-
TM57_RS09825	2104753..2105127	-	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
TM57_RS09830	2105124..2105321	-	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
TM57_RS09835	2105409..2106482	-	1074	WP_003898732.1	redox-regulated ATPase YchF	-
TM57_RS09840	2106545..2107528	+	984	WP_003898731.1	hypothetical protein	-
TM57_RS09845	2107545..2108552	-	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
TM57_RS09850	2108642..2109280	+	639	WP_003898730.1	lipid droplet-associated protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T54404 WP_003405865.1 NZ_CP011510:c2105127-2104753 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T54404 NZ_CP011510:c2105127-2104753 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT54404 WP_003405863.1 NZ_CP011510:c2105321-2105124 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT54404 NZ_CP011510:c2105321-2105124 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07