Detailed information of TA system TA054395

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1166298..1167001
• Replicon: chromosome
• Accession: NZ_CP011510
• Organism: Mycobacterium tuberculosis strain Beijing

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: TM57_RS05640
• Protein ID: WP_003409778.1
• Coordinates: 1166672..1167001 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: TM57_RS20860
• Protein ID: WP_003409780.1
• Coordinates: 1166298..1166675 (+)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TM57_RS05595	1161456..1161647	+	192	WP_003409876.1	hypothetical protein	-
TM57_RS05600	1161668..1162570	+	903	WP_069984917.1	hypothetical protein	-
TM57_RS05605	1162581..1162931	+	351	WP_003409871.1	hypothetical protein	-
TM57_RS05610	1163045..1163227	+	183	WP_003409870.1	hypothetical protein	-
TM57_RS05615	1163220..1163621	-	402	WP_003409869.1	hypothetical protein	-
TM57_RS05620	1163685..1164137	+	453	WP_003899095.1	lipoprotein	-
TM57_RS05625	1164292..1165656	-	1365	WP_003903691.1	HNH endonuclease	-
TM57_RS05630	1165711..1166301	+	591	WP_024918653.1	SEC-C domain-containing protein	-
TM57_RS20860	1166298..1166675	+	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
TM57_RS05640	1166672..1167001	+	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
TM57_RS05645	1167211..1167981	-	771	WP_003409775.1	SDR family oxidoreductase	-
TM57_RS05650	1167978..1169039	-	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
TM57_RS05655	1169036..1169551	-	516	WP_003409718.1	flavin reductase family protein	-
TM57_RS05660	1169548..1170618	-	1071	WP_003902252.1	epoxide hydrolase EphB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T54395 WP_003409778.1 NZ_CP011510:1166672-1167001 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T54395 NZ_CP011510:1166672-1167001 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT54395 WP_003409780.1 NZ_CP011510:1166298-1166675 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT54395 NZ_CP011510:1166298-1166675 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8