Detailed information of TA system TA054389

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1098479..1099105
• Replicon: chromosome
• Accession: NZ_CP011510
• Organism: Mycobacterium tuberculosis strain Beijing

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: TM57_RS05230
• Protein ID: WP_003410075.1
• Coordinates: 1098479..1098877 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A806JLL8
• Locus tag: TM57_RS05235
• Protein ID: WP_003911750.1
• Coordinates: 1098878..1099105 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TM57_RS05200	1093524..1093724	-	201	Protein_1017	hypothetical protein	-
TM57_RS05205	1094078..1095334	+	1257	WP_003902247.1	HNH endonuclease	-
TM57_RS05210	1095462..1096052	-	591	WP_003899131.1	IS110 family transposase	-
TM57_RS05215	1096006..1096653	-	648	WP_071854215.1	transposase	-
TM57_RS20825	1096714..1096893	+	180	Protein_1021	hypothetical protein	-
TM57_RS05220	1097330..1097764	-	435	WP_003914358.1	DUF1398 domain-containing protein	-
TM57_RS05225	1097865..1098296	+	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
TM57_RS05230	1098479..1098877	-	399	WP_003410075.1	PIN domain nuclease	Toxin
TM57_RS05235	1098878..1099105	-	228	WP_003911750.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
TM57_RS05240	1099364..1100533	+	1170	WP_003899126.1	ATP-binding protein	-
TM57_RS05245	1100722..1101066	+	345	WP_003410065.1	ferredoxin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T54389 WP_003410075.1 NZ_CP011510:c1098877-1098479 [Mycobacterium tuberculosis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T54389 NZ_CP011510:c1098877-1098479 [Mycobacterium tuberculosis]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8346.16 Da
• Isoelectric Point: 4.1621

>AT54389 WP_003911750.1 NZ_CP011510:c1099105-1098878 [Mycobacterium tuberculosis]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT54389 NZ_CP011510:c1099105-1098878 [Mycobacterium tuberculosis]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCAACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Structures

Toxin

• PDB: 4CHG

Antitoxin

• AlphaFold DB: A0A806JLL8