Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 499880..500511 Replicon chromosome
Accession NZ_CP011510
Organism Mycobacterium tuberculosis strain Beijing

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ28
Locus tag TM57_RS02415 Protein ID WP_003413174.1
Coordinates 499880..500257 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P95006
Locus tag TM57_RS02420 Protein ID WP_003413167.1
Coordinates 500254..500511 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
TM57_RS02380 495347..495859 + 513 WP_003413197.1 Holliday junction resolvase RuvX -
TM57_RS02385 495852..497105 + 1254 WP_003413196.1 endolytic transglycosylase MltG -
TM57_RS02390 497102..497911 + 810 WP_003413193.1 shikimate dehydrogenase -
TM57_RS02395 497923..498342 + 420 WP_003413190.1 A24 family peptidase -
TM57_RS02400 498753..498998 + 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 -
TM57_RS02405 498995..499390 + 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 -
TM57_RS02410 499490..499864 + 375 WP_003413177.1 hypothetical protein -
TM57_RS02415 499880..500257 - 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin Toxin
TM57_RS02420 500254..500511 - 258 WP_003413167.1 ribbon-helix-helix domain-containing protein Antitoxin
TM57_RS02425 500550..500963 - 414 WP_003413164.1 PIN domain nuclease -
TM57_RS21780 501056..501333 - 278 Protein_482 type II toxin-antitoxin system VapB family antitoxin -
TM57_RS02435 501330..501992 - 663 WP_003905878.1 hypothetical protein -
TM57_RS02440 501989..502648 - 660 WP_003902296.1 hypothetical protein -
TM57_RS02445 502775..503986 - 1212 WP_003900845.1 alpha/beta hydrolase family protein -
TM57_RS02450 504282..504596 - 315 WP_009937839.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13728.72 Da        Isoelectric Point: 4.4687

>T54382 WP_003413174.1 NZ_CP011510:c500257-499880 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Download         Length: 378 bp

>T54382 NZ_CP011510:c500257-499880 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9453.63 Da        Isoelectric Point: 10.6555

>AT54382 WP_003413167.1 NZ_CP011510:c500511-500254 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Download         Length: 258 bp

>AT54382 NZ_CP011510:c500511-500254 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQ28

Antitoxin

Source ID Structure
AlphaFold DB A0A829C9W5

References