Detailed information of TA system TA054378

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 299823..300502
• Replicon: chromosome
• Accession: NZ_CP011510
• Organism: Mycobacterium tuberculosis strain Beijing

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: TM57_RS01340
• Protein ID: WP_003414059.1
• Coordinates: 300086..300502 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: TM57_RS01335
• Protein ID: WP_003414061.1
• Coordinates: 299823..300089 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TM57_RS01300	295299..296036	-	738	WP_003414079.1	dienelactone hydrolase family protein	-
TM57_RS01305	296192..296992	+	801	WP_003911953.1	thymidylate synthase	-
TM57_RS01310	297063..297542	+	480	WP_003414073.1	dihydrofolate reductase	-
TM57_RS01315	297616..298035	+	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
TM57_RS01320	298032..299126	+	1095	WP_003414068.1	restriction endonuclease subunit S	-
TM57_RS01325	299136..299405	+	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
TM57_RS01330	299402..299797	+	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
TM57_RS01335	299823..300089	+	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
TM57_RS01340	300086..300502	+	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
TM57_RS01345	300589..302211	+	1623	WP_003414057.1	type I restriction-modification system subunit M	-
TM57_RS01350	302208..302483	+	276	WP_003414055.1	type I restriction endonuclease subunit S	-
TM57_RS01355	302727..303479	+	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
TM57_RS01360	303548..304450	+	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T54378 WP_003414059.1 NZ_CP011510:300086-300502 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T54378 NZ_CP011510:300086-300502 [Mycobacterium tuberculosis]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT54378 WP_003414061.1 NZ_CP011510:299823-300089 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT54378 NZ_CP011510:299823-300089 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64