Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 259935..260505 Replicon chromosome
Accession NZ_CP011510
Organism Mycobacterium tuberculosis strain Beijing

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag TM57_RS01120 Protein ID WP_003414166.1
Coordinates 260149..260505 (+) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag TM57_RS01115 Protein ID WP_003901465.1
Coordinates 259935..260165 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
TM57_RS01075 254953..255264 - 312 WP_003414190.1 hypothetical protein -
TM57_RS01080 255369..255626 - 258 WP_003899489.1 hypothetical protein -
TM57_RS01085 255860..257149 - 1290 Protein_213 transposase family protein -
TM57_RS01090 257213..257404 - 192 WP_003414184.1 hypothetical protein -
TM57_RS01095 257401..257805 - 405 WP_003414181.1 hypothetical protein -
TM57_RS21765 257953..258207 + 255 WP_003917684.1 hypothetical protein -
TM57_RS21770 258383..258658 - 276 WP_003911993.1 DUF1778 domain-containing protein -
TM57_RS01110 258849..259892 + 1044 WP_003414172.1 DUF2293 domain-containing protein -
TM57_RS01115 259935..260165 + 231 WP_003901465.1 antitoxin MazE Antitoxin
TM57_RS01120 260149..260505 + 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
TM57_RS01125 260607..262256 - 1650 WP_003899485.1 CocE/NonD family hydrolase -
TM57_RS01130 262275..262862 - 588 WP_003914429.1 DUF3558 family protein -
TM57_RS01135 263035..263361 + 327 WP_003414157.1 hypothetical protein -
TM57_RS01140 263365..265053 + 1689 WP_003414155.1 alpha/beta hydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 247616..270470 22854


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T54376 WP_003414166.1 NZ_CP011510:260149-260505 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T54376 NZ_CP011510:260149-260505 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT54376 WP_003901465.1 NZ_CP011510:259935-260165 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT54376 NZ_CP011510:259935-260165 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References