Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-StbC
Location 4207858..4208519 Replicon chromosome
Accession NZ_CP011508
Organism Pseudomonas mendocina strain NSYSU

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag PSMEN_RS19375 Protein ID WP_129481803.1
Coordinates 4208100..4208519 (+) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A8I0VLZ4
Locus tag PSMEN_RS19370 Protein ID WP_011921115.1
Coordinates 4207858..4208103 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PSMEN_RS19345 4203572..4203871 + 300 WP_148119461.1 hypothetical protein -
PSMEN_RS19350 4203871..4204197 + 327 WP_148118941.1 DUF2523 domain-containing protein -
PSMEN_RS19355 4204209..4205390 + 1182 WP_148118942.1 zonular occludens toxin -
PSMEN_RS19360 4205530..4206813 + 1284 WP_148118943.1 hypothetical protein -
PSMEN_RS19365 4206810..4207805 + 996 WP_148118944.1 tyrosine-type recombinase/integrase -
PSMEN_RS19370 4207858..4208103 + 246 WP_011921115.1 Arc family DNA-binding protein Antitoxin
PSMEN_RS19375 4208100..4208519 + 420 WP_129481803.1 type II toxin-antitoxin system VapC family toxin Toxin
PSMEN_RS19385 4208914..4209141 + 228 WP_148118945.1 hypothetical protein -
PSMEN_RS19390 4209138..4209491 - 354 WP_148118946.1 excalibur calcium-binding domain-containing protein -
PSMEN_RS24605 4209630..4209794 + 165 WP_148118947.1 hypothetical protein -
PSMEN_RS19400 4209821..4210267 + 447 WP_129481805.1 hypothetical protein -
PSMEN_RS19405 4210276..4210854 - 579 WP_148118948.1 hypothetical protein -
PSMEN_RS19410 4210956..4211867 - 912 WP_011921109.1 DMT family transporter -
PSMEN_RS19415 4212201..4212908 - 708 WP_148118949.1 DNA/RNA nuclease SfsA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-124)

Antitoxin

Arc-like DNA binding domain

(13-43)


Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 15067.30 Da        Isoelectric Point: 4.2947

>T54372 WP_129481803.1 NZ_CP011508:4208100-4208519 [Pseudomonas mendocina]
MILLDTNVLSELMRAKPAPQVLEWVDAQPVGDLVITSITVAEILYGIARMPDGKRKQGLLDVASVMFDEDFAGNILPFDA
DAAVHYAEIAAETEAKGRVVDMADAQIAAIGRLHDAVIATRNIRHFETLGVALVDPWSN

Download         Length: 420 bp

>T54372 NZ_CP011508:4208100-4208519 [Pseudomonas mendocina]
ATGATCCTGCTTGATACCAACGTGCTGTCGGAACTCATGCGTGCCAAGCCTGCACCTCAGGTTCTAGAGTGGGTTGACGC
TCAGCCTGTCGGGGATCTGGTAATCACCTCGATCACCGTTGCGGAAATTCTCTATGGCATTGCCAGGATGCCGGACGGGA
AACGCAAGCAGGGACTGCTCGATGTAGCCTCGGTCATGTTCGATGAAGACTTCGCGGGCAATATTCTGCCGTTCGATGCA
GATGCCGCTGTGCATTACGCGGAGATAGCCGCAGAAACCGAGGCTAAAGGGCGGGTGGTTGATATGGCCGATGCGCAGAT
CGCTGCAATCGGTCGGCTACATGATGCCGTGATCGCAACTCGCAATATTCGCCACTTCGAAACGCTGGGCGTTGCCCTGG
TAGATCCCTGGAGCAATTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 8990.24 Da        Isoelectric Point: 10.7217

>AT54372 WP_011921115.1 NZ_CP011508:4207858-4208103 [Pseudomonas mendocina]
MAAITIRNLDDDLKAQLRIVAASHGRSMEEEVRFIIRQALSRQEKRGGLGSRIHSRFAAVGGADLELPERKTKARAASFD
E

Download         Length: 246 bp

>AT54372 NZ_CP011508:4207858-4208103 [Pseudomonas mendocina]
ATGGCCGCTATCACTATTCGAAACCTCGACGACGACTTGAAAGCCCAGCTGCGTATTGTCGCGGCTAGCCACGGTCGCTC
CATGGAAGAAGAGGTCCGGTTCATCATTCGGCAGGCTCTGTCACGTCAAGAAAAGCGTGGCGGCTTGGGCAGTCGTATTC
ACTCCCGCTTTGCCGCTGTCGGTGGGGCTGACCTTGAGCTGCCAGAACGCAAAACCAAAGCCCGGGCAGCGAGCTTTGAT
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References