Detailed information of TA system TA054364

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 28807..29456
• Replicon: plasmid p2327
• Accession: NZ_CP011506
• Organism: Burkholderia pyrrocinia strain DSM 10685

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: ABD05_RS34865
• Protein ID: WP_011695140.1
• Coordinates: 29046..29456 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A4R4CBY5
• Locus tag: ABD05_RS34860
• Protein ID: WP_047904761.1
• Coordinates: 28807..29049 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ABD05_RS34845	25564..26529	-	966	WP_047904758.1	ParB/RepB/Spo0J family partition protein	-
ABD05_RS34850	26529..27200	-	672	WP_047904759.1	AAA family ATPase	-
ABD05_RS34855	27655..28686	+	1032	WP_047904760.1	non-homologous end-joining DNA ligase	-
ABD05_RS34860	28807..29049	+	243	WP_047904761.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
ABD05_RS34865	29046..29456	+	411	WP_011695140.1	type II toxin-antitoxin system VapC family toxin	Toxin
ABD05_RS38700	29536..29676	+	141	WP_167347872.1	hypothetical protein	-
ABD05_RS34870	29768..30115	+	348	WP_047904762.1	hypothetical protein	-
ABD05_RS34875	30164..30364	+	201	WP_047904763.1	hypothetical protein	-
ABD05_RS34880	30712..30954	+	243	WP_047904764.1	hypothetical protein	-
ABD05_RS37190	30947..32767	+	1821	Protein_27	tyrosine-type recombinase/integrase	-
ABD05_RS37195	32936..33808	+	873	WP_082146360.1	plasmid pRiA4b ORF-3 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..113484	113484

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-125)

Antitoxin

• SpoVT-AbrB domain (12-50)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14773.00 Da
• Isoelectric Point: 5.1526

>T54364 WP_011695140.1 NZ_CP011506:29046-29456 [Burkholderia pyrrocinia]
MTLYLLDTNILSNVIRDPRGACATRIGETPPEQVCTSIVVAAELRFGVWKRGSSTLAQRVEQLLASLTVLPLQPDADRCY
GRLRAELEKQGQLIGANDMLIAAHALAVDAVLVTDNTAEFTRVAGLPVENWLRSAT

Nucleotide

• Length: 411 bp

>T54364 NZ_CP011506:29046-29456 [Burkholderia pyrrocinia]
ATGACGCTGTATCTGCTCGATACCAACATTCTGTCGAACGTGATCCGCGACCCGCGCGGCGCATGCGCCACGCGGATCGG
AGAGACCCCGCCGGAGCAAGTGTGCACCTCGATCGTCGTCGCCGCGGAACTGCGGTTCGGCGTCTGGAAGCGCGGCTCGT
CAACGCTCGCGCAGCGCGTCGAGCAGCTGCTCGCGAGCCTGACCGTGTTGCCGCTGCAGCCCGACGCGGACCGGTGCTAC
GGGCGGTTGCGGGCCGAGCTCGAAAAGCAAGGTCAGCTGATCGGCGCCAACGACATGCTGATCGCCGCTCACGCGCTGGC
TGTCGACGCCGTGTTGGTCACCGACAACACGGCTGAATTCACCCGCGTCGCCGGGCTGCCGGTCGAAAACTGGCTGCGAT
CGGCGACGTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9320.56 Da
• Isoelectric Point: 4.6856

>AT54364 WP_047904761.1 NZ_CP011506:28807-29049 [Burkholderia pyrrocinia]
MPTHDTRHARLFRNGRSQAVRIPREFELPVSEVTIHREGRRLIIEPVEAAPLRELFAQWAPLDETFPDIEDLPPEPVDIE

Nucleotide

• Length: 243 bp

>AT54364 NZ_CP011506:28807-29049 [Burkholderia pyrrocinia]
ATGCCAACCCATGACACCCGCCACGCGCGCCTGTTTCGCAATGGGCGCAGCCAGGCCGTGCGGATACCGCGCGAATTCGA
GCTGCCGGTCTCGGAGGTCACCATCCACCGGGAAGGCCGCCGCTTGATCATTGAACCCGTAGAAGCGGCACCCTTGCGCG
AGCTGTTCGCTCAATGGGCCCCGCTGGACGAAACCTTTCCGGACATCGAGGACCTTCCGCCTGAACCGGTCGACATCGAA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A4R4CBY5