Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3336995..3337826 Replicon chromosome
Accession NZ_CP011495
Organism Escherichia coli strain NCM3722 isolate K-12

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag AA953_RS16745 Protein ID WP_000854814.1
Coordinates 3337452..3337826 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag AA953_RS16740 Protein ID WP_001285584.1
Coordinates 3336995..3337363 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AA953_RS16725 3334662..3336194 + 1533 WP_001350525.1 hypothetical protein -
AA953_RS26045 3336191..3336637 + 447 WP_000187523.1 RadC family protein -
AA953_RS16735 3336700..3336921 + 222 WP_000692323.1 DUF987 domain-containing protein -
AA953_RS16740 3336995..3337363 + 369 WP_001285584.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
AA953_RS16745 3337452..3337826 + 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
AA953_RS16750 3337823..3338017 + 195 WP_000988600.1 hypothetical protein -
AA953_RS26785 3338030..3338143 + 114 WP_001161660.1 DUF957 domain-containing protein -
AA953_RS25150 3338432..3338560 - 129 Protein_3171 transposase domain-containing protein -
AA953_RS16760 3338632..3338814 + 183 WP_001016348.1 hypothetical protein -
AA953_RS16765 3338915..3339244 - 330 WP_000450409.1 DUF496 family protein -
AA953_RS16770 3339416..3340474 - 1059 WP_001200891.1 FUSC family protein -
AA953_RS16775 3340672..3341145 - 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
AA953_RS16780 3341264..3342430 - 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T54334 WP_000854814.1 NZ_CP011495:3337452-3337826 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T54334 NZ_CP011495:3337452-3337826 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT54334 WP_001285584.1 NZ_CP011495:3336995-3337363 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT54334 NZ_CP011495:3336995-3337363 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References