54312

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1686363..1686981 Replicon chromosome
Accession NZ_CP011495
Organism Escherichia coli strain NCM3722 isolate K-12

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AA953_RS08295 Protein ID WP_001291435.1
Coordinates 1686363..1686581 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AA953_RS08300 Protein ID WP_000344800.1
Coordinates 1686607..1686981 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AA953_RS08265 1681652..1682224 + 573 WP_000779842.1 YbaY family lipoprotein -
AA953_RS08270 1682255..1682566 - 312 WP_000409911.1 MGMT family protein -
AA953_RS08275 1682945..1683298 + 354 WP_000878140.1 DUF1428 domain-containing protein -
AA953_RS08280 1683340..1684890 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AA953_RS08285 1685054..1685524 - 471 WP_000136192.1 YlaC family protein -
AA953_RS08290 1685640..1686191 - 552 WP_000102564.1 maltose O-acetyltransferase -
AA953_RS08295 1686363..1686581 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
AA953_RS08300 1686607..1686981 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AA953_RS08305 1687527..1690676 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
AA953_RS08310 1690699..1691892 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T54312 WP_001291435.1 NZ_CP011495:c1686581-1686363 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T54312 NZ_CP011495:c1686581-1686363 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT54312 WP_000344800.1 NZ_CP011495:c1686981-1686607 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT54312 NZ_CP011495:c1686981-1686607 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References