Detailed information of TA system TA054290

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-couple_hipB
• Location: 4316..4923
• Replicon: plasmid unnamed
• Accession: NZ_CP011480
• Organism: Hoeflea sp. IMCC20628

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A0F7PVJ5
• Locus tag: IMCC20628_RS22810
• Protein ID: WP_047032898.1
• Coordinates: 4672..4923 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: IMCC20628_RS22805
• Protein ID: WP_047032897.1
• Coordinates: 4316..4660 (-)
• Length: 115 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMCC20628_RS22790	1255..2487	-	1233	WP_047032894.1	tyrosine-type recombinase/integrase	-
IMCC20628_RS22795	2612..3568	+	957	WP_047032895.1	DUF1403 family protein	-
IMCC20628_RS22800	3565..4254	+	690	WP_156174705.1	SMC-Scp complex subunit ScpB	-
IMCC20628_RS22805	4316..4660	-	345	WP_047032897.1	helix-turn-helix transcriptional regulator	Antitoxin
IMCC20628_RS22810	4672..4923	-	252	WP_047032898.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IMCC20628_RS22815	5313..6227	-	915	WP_047032994.1	IS5 family transposase	-
IMCC20628_RS22820	6502..6789	+	288	WP_047032995.1	metalloregulator ArsR/SmtB family transcription factor	-
IMCC20628_RS22825	6786..7844	+	1059	WP_047032899.1	permease	-
IMCC20628_RS22830	7883..8119	+	237	WP_047032900.1	thioredoxin family protein	-
IMCC20628_RS22835	8236..9036	+	801	WP_197078505.1	MBL fold metallo-hydrolase	-
IMCC20628_RS22840	9036..9440	+	405	WP_047032996.1	OsmC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..154071	154071

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (13-79)

Antitoxin

• Cro/C1-type helix-turn-helix domain (58-112)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9424.98 Da
• Isoelectric Point: 10.7543

>T54290 WP_047032898.1 NZ_CP011480:c4923-4672 [Hoeflea sp. IMCC20628]
MRQISYTKMAIRVLRRMPVNKAQLIRSKIEAYAQDPASQANNVKALKGRDGIRLRVGDWRVIMDEQDNVLAILDIGPRGG
VYD

Nucleotide

• Length: 252 bp

>T54290 NZ_CP011480:c4923-4672 [Hoeflea sp. IMCC20628]
ATGAGGCAGATCAGTTATACGAAGATGGCAATCCGGGTCTTGCGCCGGATGCCAGTGAACAAGGCTCAACTGATCCGGTC
CAAGATCGAAGCCTATGCACAAGACCCAGCCTCGCAGGCGAACAATGTGAAGGCATTGAAGGGGCGAGATGGGATCCGAC
TACGCGTGGGCGATTGGCGGGTAATCATGGATGAACAAGACAATGTCCTGGCCATTCTGGATATAGGCCCCCGTGGCGGC
GTTTATGATTGA

Antitoxin

Protein

• Length: 115 a.a.
• Molecular weight: 12318.79 Da
• Isoelectric Point: 4.2350

>AT54290 WP_047032897.1 NZ_CP011480:c4660-4316 [Hoeflea sp. IMCC20628]
MNEMVTIPREEYDRLQAAAEDLADLQTYDRSKAALAAGEDELIPEGFANRLMNGESPLRVYRDLRGLTQAALAEKAGVNR
VSIAEIETGRKQGSVASLRALADALGVTLDDLAD

Nucleotide

• Length: 345 bp

>AT54290 NZ_CP011480:c4660-4316 [Hoeflea sp. IMCC20628]
ATGAACGAGATGGTGACGATTCCCCGCGAAGAATATGACCGCCTTCAGGCGGCAGCCGAAGATCTGGCCGACCTTCAAAC
CTATGATCGCTCCAAGGCGGCGCTGGCAGCGGGCGAGGACGAACTGATCCCGGAAGGCTTTGCAAACCGCCTGATGAATG
GCGAAAGCCCACTGCGCGTCTATCGCGATCTGCGCGGCTTGACCCAGGCGGCGCTGGCGGAAAAGGCCGGTGTGAACCGC
GTCTCCATTGCCGAGATAGAGACCGGTCGCAAGCAGGGGTCGGTCGCGTCCCTGCGCGCTTTGGCGGATGCCCTTGGGGT
CACACTGGACGATCTGGCTGACTGA

Structures

Toxin

• AlphaFold DB: A0A0F7PVJ5