Detailed information of TA system TA054284

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 5691602..5692307
• Replicon: chromosome
• Accession: NZ_CP011456
• Organism: Anabaena sp. WA102 isolate

Toxin (Protein)

• Gene name: tad
• Uniprot ID: A0A0K2M5N2
• Locus tag: AA650_RS25295
• Protein ID: WP_053541131.1
• Coordinates: 5691930..5692307 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: AA650_RS25290
• Protein ID: WP_053541130.1
• Coordinates: 5691602..5691925 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AA650_RS25260	5686845..5687243	+	399	WP_053541125.1	type II toxin-antitoxin system VapC family toxin	-
AA650_RS25265	5687888..5688331	-	444	Protein_5117	PIN domain-containing protein	-
AA650_RS25270	5688433..5688681	-	249	WP_053541126.1	hypothetical protein	-
AA650_RS25275	5688975..5689193	+	219	WP_053541127.1	hypothetical protein	-
AA650_RS27355	5689252..5689554	+	303	Protein_5120	DNA cytosine methyltransferase	-
AA650_RS28475	5689786..5690103	+	318	WP_168635658.1	hypothetical protein	-
AA650_RS25280	5690175..5690717	+	543	WP_053541128.1	hypothetical protein	-
AA650_RS25285	5690963..5691526	-	564	WP_053541129.1	hypothetical protein	-
AA650_RS25290	5691602..5691925	-	324	WP_053541130.1	helix-turn-helix domain-containing protein	Antitoxin
AA650_RS25295	5691930..5692307	-	378	WP_053541131.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AA650_RS25300	5692482..5693123	-	642	WP_053541132.1	hypothetical protein	-
AA650_RS25305	5693402..5694319	-	918	WP_053541133.1	NAD(+) kinase	-
AA650_RS25310	5694403..5695404	-	1002	WP_053541134.1	SDR family oxidoreductase	-
AA650_RS25315	5695722..5695826	-	105	WP_039199595.1	cytochrome b6-f complex subunit PetM	-
AA650_RS25320	5696040..5697080	+	1041	WP_053541135.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (18-110)

Antitoxin

• Cro/C1-type helix-turn-helix domain (34-89)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14332.43 Da
• Isoelectric Point: 8.4066

>T54284 WP_053541131.1 NZ_CP011456:c5692307-5691930 [Anabaena sp. WA102]
MNEKQEKPLRWIASALNDLKKFPEDVQDVMGYALDLAQHGQKHPNAKPLRGFSGAGVLEIVDDFDGDTYRAIYTVKFEGV
IYLLHSFQKKSKHGIATPKQDIELVKKRLKIAQENYLQQTTEKME

Nucleotide

• Length: 378 bp

>T54284 NZ_CP011456:c5692307-5691930 [Anabaena sp. WA102]
ATGAATGAAAAACAAGAAAAGCCTCTCAGATGGATTGCAAGCGCCCTTAATGATTTAAAGAAGTTTCCTGAAGATGTACA
GGATGTCATGGGTTACGCTCTTGATTTGGCACAACATGGACAAAAACACCCTAATGCAAAACCTTTACGCGGGTTCTCTG
GAGCAGGTGTTTTAGAAATTGTAGATGATTTTGATGGGGATACATATAGGGCAATTTATACTGTTAAATTCGAGGGTGTT
ATCTACTTACTACATTCCTTTCAAAAGAAATCAAAACATGGTATTGCTACACCAAAACAAGATATAGAATTAGTTAAAAA
AAGATTGAAAATCGCTCAAGAAAATTATTTACAACAAACTACCGAAAAAATGGAGTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11902.77 Da
• Isoelectric Point: 5.1745

>AT54284 WP_053541130.1 NZ_CP011456:c5691925-5691602 [Anabaena sp. WA102]
MPEENQVEVSSGNVFADLGLSNPEERLLKAELVRKISEIITNLNLTQVQAAEILGIDQPKVSLLIRGRLSGFSTDRLMNY
LNKLGSDVEITVKPKPENRKFAQIIVV

Nucleotide

• Length: 324 bp

>AT54284 NZ_CP011456:c5691925-5691602 [Anabaena sp. WA102]
ATGCCAGAAGAAAATCAAGTTGAGGTTAGTAGCGGAAATGTATTTGCTGACTTAGGTTTATCTAATCCTGAAGAAAGATT
ATTGAAAGCGGAGTTAGTCCGTAAAATCAGTGAAATCATTACTAATTTAAATTTAACACAAGTTCAAGCAGCAGAAATTC
TAGGCATAGATCAACCAAAAGTTTCTCTACTAATTAGAGGTAGATTAAGTGGCTTCTCAACTGATAGGTTGATGAACTAT
CTAAACAAGTTAGGTAGTGATGTAGAGATTACAGTTAAACCTAAACCTGAAAATCGTAAGTTTGCTCAGATAATTGTAGT
CTAA

Structures

Toxin

• AlphaFold DB: A0A0K2M5N2