Detailed information of TA system TA054282

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-UPF0150
• Location: 4509480..4509886
• Replicon: chromosome
• Accession: NZ_CP011456
• Organism: Anabaena sp. WA102 isolate

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0K2M315
• Locus tag: AA650_RS19840
• Protein ID: WP_053540351.1
• Coordinates: 4509704..4509886 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: AA650_RS19835
• Protein ID: WP_199924304.1
• Coordinates: 4509480..4509707 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AA650_RS19810	4504768..4507449	-	2682	WP_053540348.1	RNA helicase	-
AA650_RS19815	4507610..4508044	-	435	WP_015078603.1	DUF29 domain-containing protein	-
AA650_RS19820	4508082..4508702	-	621	WP_053541349.1	hypothetical protein	-
AA650_RS19825	4508777..4509025	+	249	WP_053540349.1	hypothetical protein	-
AA650_RS19830	4509026..4509418	+	393	WP_053540350.1	type II toxin-antitoxin system VapC family toxin	-
AA650_RS19835	4509480..4509707	-	228	WP_199924304.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
AA650_RS19840	4509704..4509886	-	183	WP_053540351.1	type II toxin-antitoxin system HicA family toxin	Toxin
AA650_RS19845	4510117..4511079	+	963	WP_053540352.1	hypothetical protein	-
AA650_RS19850	4511113..4511853	+	741	WP_053540353.1	hypothetical protein	-
AA650_RS19855	4512094..4512345	+	252	WP_053540354.1	hypothetical protein	-
AA650_RS19860	4512490..4512696	+	207	WP_053541350.1	DUF2281 domain-containing protein	-
AA650_RS26890	4512731..4512949	+	219	WP_081424280.1	DUF433 domain-containing protein	-
AA650_RS19865	4512967..4513245	+	279	WP_053540355.1	hypothetical protein	-
AA650_RS19870	4513238..4513501	+	264	WP_053540356.1	type II toxin-antitoxin system RelE/ParE family toxin	-
AA650_RS26895	4513549..4513764	-	216	Protein_4012	pyrroline-5-carboxylate reductase	-
AA650_RS19875	4513993..4514350	+	358	Protein_4013	hypothetical protein	-
AA650_RS26900	4514633..4514836	+	204	Protein_4014	HNH endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-60)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (11-66)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6691.93 Da
• Isoelectric Point: 10.6223

>T54282 WP_053540351.1 NZ_CP011456:c4509886-4509704 [Anabaena sp. WA102]
MKVKEVLKILEADGWYIDRIRGSHRILKHPSKSGIVVVPGKPSDDIPVGTLSSIWKQAKL

Nucleotide

• Length: 183 bp

>T54282 NZ_CP011456:c4509886-4509704 [Anabaena sp. WA102]
ATGAAGGTTAAAGAAGTTCTCAAAATCCTGGAAGCAGATGGTTGGTATATAGATCGCATCAGAGGTAGTCATCGTATCCT
CAAACATCCAAGCAAATCAGGTATCGTAGTTGTACCCGGAAAACCAAGTGATGATATTCCAGTCGGTACACTCTCATCAA
TTTGGAAGCAAGCAAAATTATGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8267.42 Da
• Isoelectric Point: 3.9488

>AT54282 WP_199924304.1 NZ_CP011456:c4509707-4509480 [Anabaena sp. WA102]
MNEKMIEYTVIYERGQTNWGAYVPDLPGCVSIGDTLAEVQENIKEAIALYLEVLKEDGQPIPEPSTQVGKVAVTI

Nucleotide

• Length: 228 bp

>AT54282 NZ_CP011456:c4509707-4509480 [Anabaena sp. WA102]
ATGAACGAAAAAATGATTGAGTACACAGTCATCTACGAACGTGGCCAGACAAACTGGGGCGCTTATGTTCCCGACTTACC
CGGTTGTGTCAGTATTGGCGATACCTTAGCAGAAGTACAGGAAAATATTAAAGAAGCGATCGCATTATACCTAGAAGTAT
TAAAAGAAGATGGACAACCTATACCCGAACCATCCACGCAAGTTGGTAAAGTAGCAGTAACGATATAA

Structures

Toxin

• AlphaFold DB: A0A0K2M315