Detailed information of TA system TA054281

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 3620617..3621100
• Replicon: chromosome
• Accession: NZ_CP011456
• Organism: Anabaena sp. WA102 isolate

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: AA650_RS15720
• Protein ID: WP_199924454.1
• Coordinates: 3620617..3620877 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A0K2M0Y1
• Locus tag: AA650_RS15725
• Protein ID: WP_027400594.1
• Coordinates: 3620867..3621100 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AA650_RS15700	3617259..3617675	+	417	WP_039199444.1	hypothetical protein	-
AA650_RS15705	3617689..3618087	+	399	WP_053539712.1	hypothetical protein	-
AA650_RS28040	3618192..3618572	-	381	WP_053541304.1	type II toxin-antitoxin system HicB family antitoxin	-
AA650_RS28045	3618538..3618806	-	269	Protein_3157	type II toxin-antitoxin system HicA family toxin	-
AA650_RS15715	3619068..3620501	+	1434	WP_053539713.1	tetratricopeptide repeat protein	-
AA650_RS15720	3620617..3620877	+	261	WP_199924454.1	BrnT family toxin	Toxin
AA650_RS15725	3620867..3621100	+	234	WP_027400594.1	CopG family transcriptional regulator	Antitoxin
AA650_RS15730	3621154..3621672	+	519	WP_053539715.1	DUF29 family protein	-
AA650_RS15735	3621678..3622196	+	519	WP_053539716.1	DUF29 family protein	-
AA650_RS15740	3622202..3622720	+	519	WP_039199433.1	DUF29 family protein	-
AA650_RS15745	3622726..3623244	+	519	WP_053539717.1	DUF29 family protein	-
AA650_RS15750	3623442..3624467	+	1026	WP_053539718.1	type I glyceraldehyde-3-phosphate dehydrogenase	-
AA650_RS15755	3624633..3626063	+	1431	WP_053539719.1	pyruvate kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-80)

Antitoxin

• CopG antitoxin (3-69)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10121.42 Da
• Isoelectric Point: 7.3186

>T54281 WP_199924454.1 NZ_CP011456:3620617-3620877 [Anabaena sp. WA102]
MEFEFDANKSATNKTKHNIDFIEAQKLWRDTHLVEVPANTTDEPRFLVIGKIADKHWSAVITYRSDKIRIISVRRSRTEE
VNIYES

Nucleotide

• Length: 261 bp

>T54281 NZ_CP011456:3620617-3620877 [Anabaena sp. WA102]
ATGGAATTTGAATTTGATGCAAACAAAAGTGCAACAAATAAAACTAAACATAACATTGATTTTATTGAAGCACAAAAACT
TTGGAGAGATACGCATCTAGTAGAAGTACCGGCAAACACAACCGATGAACCCAGATTTTTAGTTATTGGCAAGATAGCTG
ATAAACATTGGTCAGCAGTCATTACCTATCGTAGTGATAAAATAAGGATTATTTCTGTTCGTCGTTCTCGTACTGAAGAG
GTCAATATTTATGAAAGCTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8993.22 Da
• Isoelectric Point: 4.6263

>AT54281 WP_027400594.1 NZ_CP011456:3620867-3621100 [Anabaena sp. WA102]
MKAEEFDTKFDNGEDITEFLDLSQVHHPGYTQKRVNIDFPVWMIEALEQEAKRLGVTSDSIIKLWLAERLDKKVITN

Nucleotide

• Length: 234 bp

>AT54281 NZ_CP011456:3620867-3621100 [Anabaena sp. WA102]
ATGAAAGCTGAAGAATTTGATACTAAGTTTGACAATGGTGAAGATATTACTGAGTTTTTAGACTTATCTCAAGTACATCA
TCCAGGGTATACACAAAAAAGAGTTAATATTGATTTTCCTGTCTGGATGATAGAAGCTTTAGAACAGGAAGCAAAACGTT
TAGGTGTGACTTCTGATTCTATTATTAAATTGTGGCTTGCTGAACGTCTGGATAAAAAAGTGATTACCAATTAG

Structures

Antitoxin

• AlphaFold DB: A0A0K2M0Y1