Detailed information of TA system TA054277

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazE-MazF
• Location: 3035390..3035967
• Replicon: chromosome
• Accession: NZ_CP011456
• Organism: Anabaena sp. WA102 isolate

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A0K2M031
• Locus tag: AA650_RS13080
• Protein ID: WP_053539348.1
• Coordinates: 3035390..3035650 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: AA650_RS13085
• Protein ID: WP_053539349.1
• Coordinates: 3035638..3035967 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AA650_RS26490	3030913..3031605	-	693	WP_072033569.1	DUF3800 domain-containing protein	-
AA650_RS13060	3031880..3032608	+	729	WP_199924451.1	hypothetical protein	-
AA650_RS13065	3032724..3034391	-	1668	WP_081424219.1	hypothetical protein	-
AA650_RS13070	3034492..3034977	-	486	WP_053539346.1	DUF3368 domain-containing protein	-
AA650_RS13075	3034983..3035234	-	252	WP_053539347.1	UPF0175 family protein	-
AA650_RS13080	3035390..3035650	+	261	WP_053539348.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Toxin
AA650_RS13085	3035638..3035967	+	330	WP_053539349.1	type II toxin-antitoxin system PemK/MazF family toxin	Antitoxin
AA650_RS13090	3036115..3037884	-	1770	WP_053539350.1	pyruvate kinase	-
AA650_RS13095	3038310..3039191	+	882	WP_053539351.1	fatty acid desaturase	-
AA650_RS13100	3039237..3040106	+	870	WP_199924244.1	fructosamine kinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• SpoVT-AbrB domain (10-52)

Antitoxin

• mRNA interferase PemK-like (3-106)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9903.26 Da
• Isoelectric Point: 4.5216

>T54277 WP_053539348.1 NZ_CP011456:3035390-3035650 [Anabaena sp. WA102]
MGTAIKTRIVKIGNSQGLRIPKTLLEQSGINSEVEIEVQGNHLIIRPVEQVRKGWEKAFIEMAEKGDDILLDDINTTEWD
KDEWQW

Nucleotide

• Length: 261 bp

>T54277 NZ_CP011456:3035390-3035650 [Anabaena sp. WA102]
ATGGGTACAGCTATCAAAACCCGAATAGTCAAAATCGGTAACTCTCAAGGGTTACGCATCCCCAAAACCCTATTAGAACA
AAGTGGAATTAACTCAGAAGTAGAAATAGAAGTTCAAGGAAATCATCTCATTATTCGCCCAGTTGAACAAGTCAGAAAAG
GTTGGGAAAAAGCATTTATAGAAATGGCAGAAAAAGGCGATGATATCTTATTAGATGATATCAACACAACAGAATGGGAT
AAAGATGAATGGCAATGGTAG

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12262.38 Da
• Isoelectric Point: 7.1343

>AT54277 WP_053539349.1 NZ_CP011456:3035638-3035967 [Anabaena sp. WA102]
MAMVVNRFDVFLVNLDPTISSEIKKTRPCLIISPNEINHHISTVIVAPMTTKGQPYPTRVTCQFQGKEGQIVLDQIRTVD
KTRLIKLLGQITTEEQKAVLDILAEIFAE

Nucleotide

• Length: 330 bp

>AT54277 NZ_CP011456:3035638-3035967 [Anabaena sp. WA102]
ATGGCAATGGTAGTCAACAGATTTGATGTCTTCCTAGTTAATCTTGATCCTACCATTAGCAGTGAAATTAAAAAAACACG
CCCTTGCTTAATAATCTCACCCAACGAAATCAACCATCATATTTCTACCGTAATAGTTGCACCAATGACAACAAAAGGGC
AACCATACCCCACAAGAGTGACCTGTCAATTTCAAGGAAAAGAAGGGCAAATTGTACTTGACCAAATTCGCACAGTAGAT
AAAACTCGACTAATCAAACTTCTAGGTCAAATTACCACAGAAGAACAAAAAGCCGTTTTGGATATCTTAGCGGAAATTTT
CGCTGAATAA

Structures

Toxin

• AlphaFold DB: A0A0K2M031